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In the last years, KYN is in the spotlight in other biological processes. Here, we showed that KYN inhibited tyrosinase expression and melanin content in primary human melanocyte and keratinocyte co\u2010cultures. Furthermore, KYN decreased melanosome content in a 3D human skin reconstruction model. In these experiments, we used tyrosine + NH<jats:sub>4<\/jats:sub>Cl to induce pigmentation. We compared the inhibitory effect of KYN on melanogenesis with the already known inhibitory effect promoted by IFN\u2010\u03b3. Since increased KYN production depends on the\u00a0IFN\u2010\u03b3\u2010inducible\u00a0enzyme indoleamine\u20102,3\u2010dioxygenase (IDO), we propose that part of the effect of IFN\u2010\u03b3 on melanogenesis involves KYN production. From that, we tested if, during melanogenesis, changes in tryptophan metabolism would occur. For this purpose, we measured tryptophan, KYN and downstream products along with pigmentation. There were no significant changes in Trp metabolism, except for the high consumption of kynurenic acid. Our data identify the skin as a potential target for the action of KYN relevant for skin physiology and pigmentation. The results are discussed concerning the high production of KYN in skin inflammatory disorders and cancer.<\/jats:p>","DOI":"10.1111\/exd.14486","type":"journal-article","created":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T16:55:53Z","timestamp":1635440153000},"page":"427-432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Kynurenine inhibits melanogenesis in human melanocyte\u2010keratinocyte co\u2010cultures and in a reconstructed 3D skin model"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8163-0498","authenticated-orcid":false,"given":"Maryana Stephany","family":"Ferreira Branquinho","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Department of Clinical Chemistry and Toxicology University of Sao Paulo Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maysa Braga 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