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Here, we determined the so far unknown role of extracellular Hsp70 in epidermolysis bullosa acquisita (EBA), an anti\u2010type VII collagen autoantibody\u2010mediated blistering dermatosis. The <jats:italic>in vivo<\/jats:italic> pathophysiological relevance of extracellular Hsp70 was demonstrated in an anti\u2010type VII collagen antibody transfer\u2010induced EBA mouse model in which elevated blood levels of this chaperone were recorded. We found that Hsp70\u2010treated mice had a more intense clinical disease severity compared to controls that were paralleled by increased levels of cutaneous matrix metalloproteinase 9 and plasma hydrogen peroxide. The latter finding was confirmed in an independent reactive oxygen species release assay using EBA\u2010specific immune complexes combined with recombinant Hsp70. Finally, cell culture experiments using human naive peripheral blood mononuclear cells (PBMC) revealed that extracellular Hsp70\u00a0stimulated the secretion of the T cell\u2013derived pro\u2010inflammatory cytokines IL\u20106 and IL\u20108. This work extends knowledge about the role of Hsps in autoimmune bullous diseases, suggesting that extracellular Hsp70 represents a pathophysiological factor and potential treatment target in EBA.<\/jats:p>","DOI":"10.1111\/exd.14495","type":"journal-article","created":{"date-parts":[[2021,11,6]],"date-time":"2021-11-06T14:48:36Z","timestamp":1636210116000},"page":"528-534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evidence for a role of extracellular heat shock protein 70 in epidermolysis bullosa acquisita"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1288-0511","authenticated-orcid":false,"given":"Stefan","family":"Tukaj","sequence":"first","affiliation":[{"name":"Department of Molecular Biology Faculty of Biology University of Gda\u0144sk  Gda\u0144sk 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