{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T04:53:00Z","timestamp":1787374380011,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T00:00:00Z","timestamp":1651536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U1908206"],"award-info":[{"award-number":["U1908206"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Macrophages, which serve as a bridge between innate and adaptive immunity, play an important role in sporotrichosis. <jats:italic>Sporothrix schenckii<\/jats:italic> infections can produce immune responses such as macrophage polarization and inflammatory factor secretion. In the early stages of inflammation, the expression of DAB2 in macrophages is increased, which controls the secretion of inflammatory factors and affects the polarization of macrophages. However, the expressions and mechanisms of DAB2 in sporotrichosis are not clear. In this study, we examined the expression of DAB2 and its regulation of inflammatory factors under conditions of <jats:italic>Sporothrix schenckii<\/jats:italic> infection. Our results indicated that the <jats:italic>Sporothrix schenckii<\/jats:italic> infection increased the expression of DAB2 and revealed a mixed M1\/M2\u2010like type of gene expression in BMDMs with the inhibited <jats:italic>Il<\/jats:italic>\u2010<jats:italic>6<\/jats:italic>, <jats:italic>Il1<\/jats:italic>\u2010<jats:italic>\u03b2<\/jats:italic> and <jats:italic>Arg<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> and induced <jats:italic>Tnf<\/jats:italic>\u2010<jats:italic>\u03b1<\/jats:italic>, <jats:italic>Il<\/jats:italic>\u2010<jats:italic>10<\/jats:italic> and <jats:italic>Mgl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic>. The deficiency of <jats:italic>Dab2<\/jats:italic>\u00a0gene suspended the changes of cytokines. In addition, JNK activity in BMDMs was inhibited by <jats:italic>Sporothrix schenckii<\/jats:italic> infection, leading to an increase in c\u2010JUN. We also identified c\u2010JUN as a transcription factor for <jats:italic>Dab2<\/jats:italic> through chromatin immunoprecipitation and luciferase reporter assays. In an <jats:italic>in vivo<\/jats:italic> mouse model, sporotrichosis\u2010induced skin lesions were accompanied with an upregulation of c\u2010JUN and inhibition of JNK activity, which were in accord with findings from <jats:italic>in vitro<\/jats:italic> experiments. Taken together, these findings indicate that in the early stages of <jats:italic>Sporothrix schenckii<\/jats:italic> infection there is a promotion of DAB2 expression through the JNK\/c\u2010JUN pathway, effects that can then control the expression of inflammatory factors.<\/jats:p>","DOI":"10.1111\/exd.14580","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T09:48:29Z","timestamp":1650448109000},"page":"1330-1340","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Sporothrix schenckii<\/i> regulates macrophage inflammatory responses via the c\u2010JUN\u2010induced <i>Dab2<\/i> transcription"],"prefix":"10.1111","volume":"31","author":[{"given":"Shengnan","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Dermatology The First Hospital of China Medical University  Shenyang China"},{"name":"Key Laboratory of Immunodermatology Ministry of Health and Ministry 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