{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T08:27:14Z","timestamp":1768292834411,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20ek0109488"],"award-info":[{"award-number":["JP20ek0109488"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20ek0109450h0001"],"award-info":[{"award-number":["JP20ek0109450h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013328","name":"Research Organization of Information and Systems","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013328","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Non\u2010melanoma skin cancer (NMSC) is mainly caused by ultraviolet (UV)\u2010induced somatic mutations and is characterized by UV signature modifications. Xeroderma pigmentosum group A (<jats:italic>Xpa<\/jats:italic>) knockout mice exhibit extreme UV\u2010induced photo\u2010skin carcinogenesis, along with a photosensitive phenotype. We performed whole\u2010exome sequencing (WES) of squamous cell carcinoma (SCC) samples after repetitive ultraviolet B (UVB) exposure to investigate the differences in the landscape of somatic mutations between <jats:italic>Xpa<\/jats:italic> knockout and wild\u2010type mice. Although the tumors that developed in mice harboured UV signature mutations in a similar set of cancer\u2010related genes, the pattern of transcriptional strand asymmetry was largely different; UV signature mutations in <jats:italic>Xpa<\/jats:italic> knockout and wild\u2010type mice preferentially occurred in transcribed and non\u2010transcribed strands, respectively, reflecting a deficiency in transcription\u2010coupled nucleotide excision repair in <jats:italic>Xpa<\/jats:italic> knockout mice. Serial time point analyses of WES for a tumor induced by only a single UVB exposure showed pathogenic mutations in <jats:italic>Kras<\/jats:italic>, <jats:italic>Fat1<\/jats:italic>, and <jats:italic>Kmt2c<\/jats:italic>, which may be driver genes for the initiation and promotion of SCC in <jats:italic>Xpa<\/jats:italic> knockout mice. Furthermore, the inhibitory effects on tumor production in <jats:italic>Xpa<\/jats:italic> knockout mice by the anti\u2010inflammatory CXCL1 monoclonal antibody affected the pattern of somatic mutations, wherein the transcriptional strand asymmetry was attenuated and the activated signal transduction was shifted from the RAS\/RAF\/MAPK to the PIK3CA pathway.<\/jats:p>","DOI":"10.1111\/exd.14634","type":"journal-article","created":{"date-parts":[[2022,6,25]],"date-time":"2022-06-25T12:24:47Z","timestamp":1656159887000},"page":"1607-1617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The landscape of genetic alterations of <scp>UVB<\/scp>\u2010induced skin tumors in <scp>DNA<\/scp> repair\u2010deficient mice"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6534-4566","authenticated-orcid":false,"given":"Ai","family":"Yoshioka","sequence":"first","affiliation":[{"name":"Division of Dermatology, Department of Internal Related, Graduate School of Medicine Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8454-1159","authenticated-orcid":false,"given":"Hirofumi","family":"Nakaoka","sequence":"additional","affiliation":[{"name":"Department of Cancer Genome Research Sasaki Institute  Tokyo Japan"},{"name":"Human Genetics Laboratory National Institute of Genetics  Mishima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0364-711X","authenticated-orcid":false,"given":"Takeshi","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Internal Related, Graduate School of Medicine Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1706-8493","authenticated-orcid":false,"given":"Ituro","family":"Inoue","sequence":"additional","affiliation":[{"name":"Human Genetics Laboratory National Institute of Genetics  Mishima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6784-2849","authenticated-orcid":false,"given":"Chikako","family":"Nishigori","sequence":"additional","affiliation":[{"name":"Division of Research on Intractable Dermatological Disease, Department of iPS cell Applications Kobe University Graduate School of Medicine  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2210-6108","authenticated-orcid":false,"given":"Makoto","family":"Kunisada","sequence":"additional","affiliation":[{"name":"Division of Dermatology, Department of Internal Related, Graduate School of Medicine Kobe University  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2014.08.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1269\/jrr.10175"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.120"},{"issue":"5","key":"e_1_2_9_5_1","first-page":"1178","article-title":"The pathogenesis of squamous cell cancer: lessons learned from studies of skin carcinogenesis\u2010\u2010thirty\u2010third G. H. a. Clowes memorial award lecture","volume":"54","author":"Yuspa SH","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.22.10124"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940040306"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.19.10354"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1099"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.154"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa6806"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2010.083022"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/377165a0"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206023"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12365625"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0724"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2017.04.034"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5701001"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.128"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14507"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.07.037"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28568-2"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1943-3"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.08.042"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-1768"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.04.004"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12377"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/ges029"},{"issue":"4","key":"e_1_2_9_29_1","first-page":"641","article-title":"Strand specificity and absence of hot spots for p53 mutations in ultraviolet B\u2010induced skin tumors of XPA\u2010deficient mice","volume":"58","author":"Takeuchi S","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(01)00093-8"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201744"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2015.01.013"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1975.tb06650.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/372773a0"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.12.008"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206096"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.78.93"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2017.00019"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13052"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aao4426"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.01.018"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.06.009"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.245"},{"issue":"9","key":"e_1_2_9_44_1","first-page":"3065","article-title":"Inhibition of mTORC1 leads to MAPK pathway activation through a PI3K\u2010dependent feedback loop in human cancer","volume":"118","author":"Carracedo A","year":"2008","journal-title":"J Clin Invest"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.10.001"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601911408079"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2010.03.007"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14634","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.14634","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T07:24:27Z","timestamp":1692602667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.14634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,7]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/exd.14634"],"URL":"https:\/\/doi.org\/10.1111\/exd.14634","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,7]]},"assertion":[{"value":"2022-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}