{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:28:09Z","timestamp":1790011689622,"version":"4.0.1"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T00:00:00Z","timestamp":1660867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008271","name":"LEO Pharma Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Psoriasis is a chronic inflammatory disease whereby long\u2010term disease control remains a challenge for the patients. Latest evidence suggests that combined topical treatment with steroids and vitamin D analogue foam (Calcipotriol\/Betamethasone) is efficient in long\u2010term management of the disease and reducing the number of relapses. Its effects on cellular inflammation and cytokine production remain to be explored. We set out to examine the effect of topical therapies on cellular infiltrate and cytokine profile in the lesional skin of psoriasis patients. This was a monocentric, double\u2010blind, randomized trial with 30 patients. Patients were treated with the combined Calcipotriol\/Betamethasone foam, Betamethasone foam alone, Clobetasol Propionate ointment or placebo. 4\u2009mm skin biopsies from lesional and non\u2010lesional sites were taken before and 4\u2009weeks after treatment. Cellular infiltrate, IFN\u03b3 and IL\u201017 were studied by immunofluorescence. Each patient was their own control. Evolution in skin inflammation was studied in parallel with changes in patient's epidermal thickness and their tPASI clinical score. Lesional skin was characterized by increased epidermal thickness, increased number of IL\u201017 and IFN\u03b3 producing CD8+ T cells, NK cells and neutrophils. All treatment reduced epidermal thickness and improved patients tPASI scores. Only the combined Calcipotriol\/Betamethasone foam completely abolished epidermal and dermal influx of CD8+ T cells, reduced number of CD8\u2009+\u2009IFN\u03b3+ cells (but not CD8\u2009+\u2009IL\u201017+ cells) and significantly reduced the number of MPO+ neutrophils which were predominantly IL\u201017+. None of the treatments had effect on NK cells. We have shown the combined topical treatment with Calcipotriol\/Betamethasone foam to be effective in reducing cellular influx into lesional skin of psoriasis patients and this effect to be superior to emollient or Betamethasone alone. Its previously described efficacy in the clinic may be attributed to its unique and rapid ability to inhibit both adaptive CD8+ T cell and innate immune neutrophilia influx into the skin, which was not observed for the other treatments.<\/jats:p>","DOI":"10.1111\/exd.14657","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T14:39:58Z","timestamp":1660055998000},"page":"1764-1778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Impact of topical emollient, steroids alone or combined with calcipotriol, on the immune infiltrate and clinical outcome in psoriasis"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7858-4822","authenticated-orcid":false,"given":"Marjorie","family":"Heim","sequence":"first","affiliation":[{"name":"Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Universit\u00e9 C\u00f4te d'Azur  Nice 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