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However, most of them use single\u2010cell or reconstructed human skin models that did not have complex anatomy of human skin. Thus, this study aimed to create a new model of psoriasis\u2010like dermatitis using human skin under in vitro conditions. To perform this, human skin explants were topically treated with imiquimod (IMQ) or vehicle for 2, 3 or 6 consecutive days. Some explants were treated with an anti\u2010psoriatic drug or antibody anti\u2010interleukin\u201017A (IL\u201017A). Topical application of IMQ increased total epidermal area, epidermal proliferation and keratinocyte differentiation at 3, 4 or 7\u2009days. The protein levels of CD3 were augmented in the IMQ\u2010treated human skin explants at 7\u2009days reflecting the activation of T cells. Topical IMQ promoted a higher protein and mRNA levels of IL\u201017A in human skin ex vivo. Immunofluorescence analysis showed CD207\u2010positive Langerhans cells (LCs) and CD3\u2010positive T cells expressing IL\u201017A in IMQ\u2010treated human skin explants at 7\u2009days. In addition, administration of antibody anti\u2010IL\u201017A or an anti\u2010psoriatic drug inhibited IMQ\u2010induced increase in the epidermal thickness in ex vivo human skin at 7\u2009days. In conclusion, topical IMQ application promotes epidermal changes in ex vivo human skin that resemble to human psoriatic skin lesions. Moreover, IMQ\u2010induced production of IL\u201017 by LCs and T cells is critical to development of psoriasis\u2010like inflammation in our model. This new model is suitable for in vitro screening of antipsoriatic drugs.<\/jats:p>","DOI":"10.1111\/exd.14659","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T05:46:46Z","timestamp":1660283206000},"page":"1791-1799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Imiquimod\u2010induced ex vivo model of psoriatic human skin via <scp>interleukin\u201017A<\/scp> signalling of T cells and Langerhans cells"],"prefix":"10.1111","volume":"31","author":[{"given":"Eduarda Cristina da Silva","family":"Carlos","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology Rio de Janeiro State University  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielly Alves","family":"Cristov\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Rio de Janeiro State University  Rio de Janeiro 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