{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:29:37Z","timestamp":1793255377781,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,8,26]],"date-time":"2022-08-26T00:00:00Z","timestamp":1661472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Traditional high\u2010frequency ultrasound (HFUS; 20\u2009MHz) is a non\u2010invasive method used to study skin in vivo but is not able to measure skin thickness accurately and to identify the dermal\u2010epidermal junction (DEJ). Ultra\u2010high frequency ultrasound (UHFUS; 70\u2013100\u2009MHz) has sub\u2010millimetre resolution comparable to histology. The aim of this study was to identify, by UHFUS, the DEJ and to describe skin differences in healthy individuals by providing a measure of skin thickness, based on age and gender. We also described the bullous pemphigoid lesion. We enrolled 42 patients divided into 2 groups: A and B. Group A included 32 healthy volunteers aged 22\u201374\u2009years. Group B consisted of 10 patients with bullous pemphigoid. For each patient in group A, 8 ultrasound (US) clips by 70\u2009MHz probe were performed at forehead, cheek, nose, forearm, abdomen, chest, back and leg. For each patient in group B, 5 US images were acquired at blisters roofs and edges. In each US image, we measured thickness of stratum corneum (\u03b1\u2010\u03b2), epidermis (\u03b1\u2010\u03b3) and epidermis plus dermis (\u03b1\u2010\u03b4). In both groups, we found the presence of 4 lines delimiting: the stratum corneum (the layer between \u03b1\u2010line and \u03b2\u2010line), the epidermis (distance between \u03b1\u2010 and \u03b3\u2010line), and the boundary between dermis and subcutis (\u03b4\u2010line). The \u03b3\u2010line corresponds to the point of detachment of the bullae. The abdominal \u03b1\u2010\u03b2 layer was thicker in males (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.019) and \u03b1\u2010\u03b4 thickness at cheeks (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), chest (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.007), back (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.025) and forearm (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In females, \u03b1\u2010\u03b3 thickness of the back was greater (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.005). In old people compared to young, we noticed an increase of \u03b1\u2010\u03b2 layer at forehead and chest (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.014), an increase of \u03b1\u2010\u03b3 layer at forearm (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.001), back (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.024) and leg (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.010) and an increase of \u03b1\u2010\u03b4 layer at forehead (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.001) and nose (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.049). UHFUS is an advanced imaging technique that can detect both the DEJ and the boundary between dermis and subcutaneous tissue so that epidermal and dermal thickness can be measured with good accuracy. Regarding gender and age, skin differences obtained with UHFUS were comparable to other non\u2010invasive methods.<\/jats:p>","DOI":"10.1111\/exd.14664","type":"journal-article","created":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T07:08:20Z","timestamp":1661238500000},"page":"1863-1871","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Ultra\u2010high frequency ultrasound detection of the dermo\u2010epidermal junction: Its potential role in dermatology"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6788-3253","authenticated-orcid":false,"given":"Giammarco","family":"Granieri","sequence":"first","affiliation":[{"name":"Department of Dermatology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7277-3278","authenticated-orcid":false,"given":"Teresa","family":"Oranges","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Meyer Childern's University Hospital  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Morganti","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Janowska","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Romanelli","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Manni","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Dini","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,26]]},"reference":[{"issue":"536","key":"e_1_2_12_2_1","first-page":"611","article-title":"Epidermal and dermal thickness","volume":"45","author":"Whitton JT","year":"1972","journal-title":"Br J 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