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Skin explants (<jats:italic>n<\/jats:italic>\u2009=\u20096) were maintained in the 3D\u2010SeboSkin model, in direct contact to fibroblasts and alone in serum\u2010free medium (SFM). Histopathological, immunohistochemical, apoptosis and oil red staining evaluations were performed at Days 0 and 6 of incubation. Results revealed preservation and prominent proliferation of basal keratinocytes of the skin explants in addition to preservation of dermal collagen and vasculature at Day 6 in the 3D\u2010SeboSkin culture model and to a lesser extent in co\u2010culture with fibroblasts but not in SFM alone. Melan\u2010A+\/Ki67\u2010 epidermal melanocytes remained attached to the dermis even at sites of epidermal detachment in the three skin explant models tested. However, the number of epidermal melanocytes was significantly conserved in 3D\u2010SeboSkin cultures in comparison with skin explants in SFM (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), whereas no difference was found in comparison with the co\u2010culture with fibroblasts. Few DAPI\/TUNEL+ apoptotic melanocytes could mostly be observed in SFM\u2010incubated skin explants. Furthermore, only SZ95 sebocytes in contact to skin explants in 3D\u2010SeboSkin exhibited increased lipogenesis with accumulation of abundant lipid droplets. These results denote that the 3D\u2010SeboSkin model yielded significant preservation of epidermal melanocytes and hence it is optimal for ex vivo studies of abnormalities of skin pigmentation, melanocyte neoplasms and effects of different hormones, cytokines, carcinogens and various therapeutics in a pattern that recapitulates the in vivo environment.<\/jats:p>","DOI":"10.1111\/exd.14813","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T04:44:02Z","timestamp":1686199442000},"page":"1063-1071","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Preservation of epidermal melanocyte integrity in an ex vivo co\u2010culture skin model with sebocytes"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6406-6223","authenticated-orcid":false,"given":"Mohamed Badawy","family":"Abdel\u2010Naser","sequence":"first","affiliation":[{"name":"Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg  Dessau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0920-9092","authenticated-orcid":false,"given":"Georgios","family":"Nikolakis","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg  Dessau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1646-2608","authenticated-orcid":false,"given":"Christos C.","family":"Zouboulis","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg  Dessau Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Abdel\u2010NaserMB AbdallahM deAlmeidaHL JrWU.Human skin cell culture and its impact on Dermatology.2005https:\/\/www.researchgate.net\/publication\/234076582_Human_Skin_Cell_Culture_and_its_Impact_on_Dermatology. 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