{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:41:41Z","timestamp":1788997301010,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003503","name":"Nederlandse Brandwonden Stichting","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Wound healing of deep burn injuries is often accompanied by severe scarring, such as hypertrophic scar (HTS) formation. In severe burn wounds, where the subcutis is also damaged, the scars adhere to structures underneath, resulting in stiffness of the scar and impaired motion. Over the recent years, a promising solution has emerged: autologous fat grafting, also known as <jats:italic>lipofilling<\/jats:italic>. Previous clinical reports have shown that the anti\u2010fibrotic effect has been attributed to the presence of adipose\u2010derived stromal cells (ADSC). In the proposed study, we aim to investigate the effect of fat grafting in 3D organotypic skin cultures mimicking an HTS\u2010like environment. To this end, organotypic skin cultures were embedded with normal skin fibroblasts (NF) or HTS\u2010derived fibroblasts with or without incorporation of human adipose subcutaneous tissue (ADT) and one part was thermally wounded to examine their effect on epithelialization. The developed skin cultures were analysed on morphology and protein level. Analysis revealed that ADT\u2010containing organotypic skin cultures comprise an improved epidermal homeostasis, and a fully formed basement membrane, similar to native human skin (NHS). Furthermore, the addition of ADT significantly reduced myofibroblast presence, which indicates its anti\u2010fibrotic effect. Finally, re\u2010epithelialization measurements showed that ADT reduced re\u2010epithelialization in skin cultures embedded with NFs, whereas HTS\u2010fibroblast\u2010embedded skin cultures showed complete wound closure. In conclusion, we succeeded in developing a 3D organotypic HTS\u2010skin model incorporated with subcutaneous tissue that allows further investigation on the molecular mechanism of fat grafting.<\/jats:p>","DOI":"10.1111\/exd.14893","type":"journal-article","created":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T06:59:59Z","timestamp":1690613999000},"page":"1752-1762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mimicking fat grafting of fibrotic scars using 3D\u2010organotypic skin cultures"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4168-6443","authenticated-orcid":false,"given":"Rajiv","family":"Raktoe","sequence":"first","affiliation":[{"name":"Department of Dermatology Leiden University Medical Centre (LUMC)  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia K. A. 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