{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T12:19:29Z","timestamp":1793881169457,"version":"4.7.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mast cells have traditionally been associated with allergic inflammatory responses; however, they play important roles in cutaneous innate immunity and wound healing. The Hidradenitis Suppurativa tissue transcriptome is associated with alterations in innate immunity and wound healing\u2010associated pathways; however, the role of mast cells in the disease is unexplored. We demonstrate that mast cell\u2010associated gene expression (using whole tissue RNAseq) is upregulated, and in\u2010silico cellular deconvolution identifies activated mast cells upregulated and resting mast cells downregulated in lesional tissue. Tryptase\/Chymase positive mast cells (identified using IHC) localize adjacent to epithelialized tunnels, fibrotic regions of the dermis and at perivascular sites associated with Neutrophil Extracellular Trap formation and TNF\u2010alpha production. Treatment with Spleen Tyrosine Kinase antagonist (Fostamatinib) reduces the expression of mast cell\u2010associated gene transcripts, associated biochemical pathways and the number of tryptase\/chymase positive mast cells in lesional hidradenitis suppurativa tissue. This data indicates that although mast cells are not the most abundant cell type in Hidradenitis Suppurativa tissue, the dysregulation of mast cells is paralleled with B cell\/plasma cell inflammation, inflammatory epithelialized tunnels and epithelial budding. This provides an explanation as to the mixed inflammatory activation signature seen in HS, the correlation with dysregulated wound healing and potential pathways involved in the development of epithelialized tunnels.<\/jats:p>","DOI":"10.1111\/exd.14894","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T09:10:01Z","timestamp":1690794601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mast cells are upregulated in hidradenitis suppurativa tissue, associated with epithelialized tunnels and normalized by spleen tyrosine kinase antagonism"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7031-2710","authenticated-orcid":false,"given":"A.","family":"Flora","sequence":"first","affiliation":[{"name":"Laboratory of Translational Cutaneous Medicine Ingham Institute for Applied Medical Research  Sydney New South Wales Australia"},{"name":"University of New South Wales  Sydney New South Wales Australia"},{"name":"Department of Dermatology Liverpool Hospital  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7443-8748","authenticated-orcid":false,"given":"R.","family":"Jepsen","sequence":"additional","affiliation":[{"name":"Holdsworth House Medical Practice  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8311-9899","authenticated-orcid":false,"given":"E. K.","family":"Kozera","sequence":"additional","affiliation":[{"name":"University of New South Wales  Sydney New South Wales Australia"},{"name":"Department of Dermatology Liverpool Hospital  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-2702","authenticated-orcid":false,"given":"J. A.","family":"Woods","sequence":"additional","affiliation":[{"name":"University of New South Wales  Sydney New South Wales Australia"},{"name":"Department of Dermatology Liverpool Hospital  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7775-1502","authenticated-orcid":false,"given":"G. D.","family":"Cains","sequence":"additional","affiliation":[{"name":"University of New South Wales  Sydney New South Wales Australia"},{"name":"Department of Dermatology Liverpool Hospital  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7539-5479","authenticated-orcid":false,"given":"M.","family":"Radzieta","sequence":"additional","affiliation":[{"name":"South West Sydney Limb Preservation and Wound Research Ingham Institute for Applied Medical Research  Sydney New South Wales Australia"},{"name":"School of Medicine Western Sydney University  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4489-589X","authenticated-orcid":false,"given":"S. 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