{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:41:45Z","timestamp":1788907305501,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:00:00Z","timestamp":1692576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Corticotropin\u2010releasing hormone receptor\u20101 (CRH\u2010R1) is expressed in human mast cells, but its role in skin diseases is unknown. By using a sequential double\u2010staining technique, the mast cell expression of CRH\u2010R1 was investigated in biopsies from lesional and non\u2010lesional skin samples of patients with actinic keratosis (AK), basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and psoriasis. Dermal tryptase<jats:sup>+<\/jats:sup> mast cells expressed CRH\u2010R1 immunoreactivity in the non\u2010lesional skin in all patient groups. The CRH\u2010R1 expression was significantly increased in the lesional skin of AK (<jats:italic>p<\/jats:italic>\u2009=\u20090.03) and psoriasis (<jats:italic>p<\/jats:italic>\u2009=\u20090.02), non\u2010significantly in BCC (<jats:italic>p<\/jats:italic>\u2009=\u20090.129), but not increased in SCC. To investigate the regulation of CRH\u2010R1, the LAD2 mast cell line was irradiated with UVB or stimulated with CRH or 1,25\u2010dihydroxyvitamin D<jats:sub>3<\/jats:sub> [1,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub>]. Consequently, UVB at 90\u2009mJ\/cm<jats:sup>2<\/jats:sup> (<jats:italic>p<\/jats:italic>\u2009=\u20090.041) and 120\u2009mJ\/cm<jats:sup>2<\/jats:sup> (<jats:italic>p<\/jats:italic>\u2009=\u20090.039) decreased CRH\u2010R1 expression. Instead, CRH at 100 and 1000\u2009nM increased CRH\u2010R1 immunostaining, but did not affect the proliferative response. The treatment with 10 and 100\u2009nM 1,25\u2010(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> led to a noticeable increase in CRH\u2010R1 staining. After irradiating with UVB, the concentration of CRH increased in the conditioned medium, but not in sonicated LAD2 mast cells. In conclusion, the lack of sufficient levels of CRH\u2010R1 in mast cells may be related to diminished antitumoural response in SCC and possibly in BCC.<\/jats:p>","DOI":"10.1111\/exd.14903","type":"journal-article","created":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T03:09:51Z","timestamp":1692673791000},"page":"1794-1804","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Corticotropin\u2010releasing hormone receptor\u20101 is increased in mast cells in psoriasis and actinic keratosis, but not markedly in keratinocyte skin carcinomas"],"prefix":"10.1111","volume":"32","author":[{"given":"Salla","family":"Haimakainen","sequence":"first","affiliation":[{"name":"Department of Dermatology Kuopio University Hospital and University of Eastern Finland  Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9504-7255","authenticated-orcid":false,"given":"Ilkka T.","family":"Harvima","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kuopio University Hospital and University of Eastern Finland  Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,21]]},"reference":[{"issue":"2","key":"e_1_2_10_2_1","first-page":"48","article-title":"Epidemiology of skin cancer","volume":"23","author":"Boni R","year":"2002","journal-title":"Neuro Endocrinol Lett"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13107"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000350757"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2012.01438.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2009.02.001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13402"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800474"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1197"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08686.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.56"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00217.2011"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2020.03.008"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0805"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.12.7665"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","unstructured":"TheoharidesTC SinghLK BoucherW et al.Corticotropin\u2010releasing hormone induces skin mast cell degranulation and increased vascular permeability a possible explanation for its proinflammatory effects*[Internet].1998. Accessed February 15 2019.https:\/\/academic.oup.com\/endo\/article\u2010abstract\/139\/1\/403\/2987512","DOI":"10.1210\/en.139.1.403"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467209666161026144219"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07442.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsp.5640192"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357907.2017.1289216"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2015.2645"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357907.2019.1565765"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-007-0290-7"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.02760.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(02)00343-0"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24274"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.334"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0487fje"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2017-03230"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2013.02.006"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.01.041"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1586\/eci.12.1"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.05.001"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2016.08.006"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2017.3132"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14903","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,6]],"date-time":"2025-04-06T03:38:15Z","timestamp":1743910695000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.14903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,21]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1111\/exd.14903"],"URL":"https:\/\/doi.org\/10.1111\/exd.14903","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,21]]},"assertion":[{"value":"2023-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}