{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:16:25Z","timestamp":1778588185580,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T00:00:00Z","timestamp":1699142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Psoriasis is a persistent inflammatory skin disease thought to arise as a result of the infiltration of inflammatory cells and activation of keratinocytes. Recent advances in basic research and clinical experience revealed that the interleukin (IL)\u201023\/IL\u201017 axis has been identified as a major immune pathway in psoriasis. However, it remains unclear how keratinocyte factors contribute to the pathology of psoriasis. Keratinocyte proline\u2010rich protein (KPRP) is a proline\u2010rich insoluble protein, which is present in the epidermis and is likely to be involved in the skin barrier function. Here, to investigate the potential roles of KPRP in psoriatic skin inflammation, <jats:italic>Kprp<\/jats:italic>\u2010modified mice were applied in the imiquimod (IMQ)\u2010induced skin inflammation model, which develops psoriasis\u2010like epidermal hyperplasia and cutaneous inflammation features. Then, heterozygous knockout (<jats:italic>Kprp<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup>) but not homozygous knockout (<jats:italic>Kprp<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>) mice displayed attenuated skin erythema compared to control wild\u2010type mice. In addition, RNA sequencing, quantitative PCR and\/or histological analysis detected changes in the expression of several molecules related to psoriatic inflammation or keratinocyte differentiation in <jats:italic>Kprp<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> mice, but not <jats:italic>Kprp<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice. Further analysis exhibited reduced IL\u201017\u2010producing \u03b3\u03b4<jats:sup>low<\/jats:sup> T cells and amplified epidermal hyperplasia in <jats:italic>Kprp<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> mice, which were implied to be related to decreased expression of \u03b2\u2010defensins and increased expression of LPAR1 (Lysophosphatidic acid receptor 1), respectively. Thus, our results imply that KPRP has the potential as a therapeutic target in psoriatic skin inflammation.<\/jats:p>","DOI":"10.1111\/exd.14960","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T05:38:57Z","timestamp":1699249137000},"page":"2121-2130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Keratinocyte proline\u2010rich protein modulates immune and epidermal response in imiquimod\u2010induced psoriatic skin inflammation"],"prefix":"10.1111","volume":"32","author":[{"given":"Ai","family":"Matsuno","sequence":"first","affiliation":[{"name":"Department of Dermatology, Graduate School of Medicine The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0531-559X","authenticated-orcid":false,"given":"Hayakazu","family":"Sumida","sequence":"additional","affiliation":[{"name":"Department of 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