{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:27:58Z","timestamp":1792729678811,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703099"],"award-info":[{"award-number":["81703099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81903212"],"award-info":[{"award-number":["81903212"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018557","name":"Science and Technology Project of Nantong City","doi-asserted-by":"publisher","award":["MS12021014"],"award-info":[{"award-number":["MS12021014"]}],"id":[{"id":"10.13039\/501100018557","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    An excessive proliferation of skin fibroblasts usually results in different skin fibrotic diseases. Hydrogen sulphide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) is regarded as an important endogenous gasotransmitter with various functions. The study aimed to investigate the roles and mechanisms of H\n                    <jats:sub>2<\/jats:sub>\n                    S on primary mice skin fibroblasts proliferation. Cell proliferation and collagen synthesis were assessed with the expression of \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), proliferating cell nuclear antigen (PCNA), Collagen I and Collagen III. The degree of oxidative stress was evaluated by dihydroethidium (DHE) and MitoSOX staining. Mitochondrial membrane potential (\u0394\u03a8m) was detected by JC\u20101 staining. Necroptosis was evaluated with TDT\u2010mediated dUTP nick end labelling (TUNEL) and expression of receptor\u2010interacting protein kinase 1 (RIPK1), RIPK3 and mixed lineage kinase domain\u2010like protein (MLKL). The present study found that \u03b1\u2010SMA, PCNA, Collagen I and Collagen III expression were increased, oxidative stress was promoted, \u0394\u03a8m was impaired and positive rate of TUNEL staining, RIPK1 and RIPK3 expression as well as MLKL phosphorylation were all enhanced in skin fibroblasts from cystathionine \u03b3\u2010lyase (CSE) knockout (KO) mice or transforming growth factor\u2010\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    (TGF\u2010\u03b2\n                    <jats:sub>1,<\/jats:sub>\n                    10\u2009ng\/mL)\u2010stimulated mice skin fibroblasts, which was restored by exogenous sodium hydrosulphide (NaHS, 50\u2009\u03bcmol\/L). In conclusion, endogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S production impairment in CSE\u2010deficient mice accelerated skin fibroblasts proliferation via promoted necroptosis, which was attenuated by exogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S. Exogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S supplement alleviated proliferation of skin fibroblasts with TGF\u2010\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    stimulation via necroptosis inhibition. This study provides evidence for H\n                    <jats:sub>2<\/jats:sub>\n                    S as a candidate agent to prevent and treat skin fibrotic diseases.\n                  <\/jats:p>","DOI":"10.1111\/exd.14972","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T22:49:02Z","timestamp":1700261342000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Endogenous hydrogen sulphide deficiency and exogenous hydrogen sulphide supplement regulate skin fibroblasts proliferation via necroptosis"],"prefix":"10.1111","volume":"33","author":[{"given":"Ling","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"},{"name":"Yancheng First Hospital, Affiliated Hospital of Nanjing University Medical School The First people's Hospital of Yancheng  Yancheng China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziying","family":"He","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyan","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuanglin","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Affiliated Hospital of Nantong University Medical School of Nantong University  Nantong 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