{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:13:55Z","timestamp":1789665235305,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzen Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Generalized pustular psoriasis (GPP) is considered to be a distinct clinical entity from psoriasis vulgaris (PV), with different clinical and histological manifestations. The pathogenesis of GPP has not been thoroughly elucidated, especially in those patients lacking interleukin (IL)36RN. In present study, we performed RNA sequence analysis on skin lesions from 10 GPP patients (4 with and 6 without <jats:italic>IL36RN<\/jats:italic> mutation) and 10 PV patients without <jats:italic>IL36RN<\/jats:italic> mutation. Compared with PV, significantly overexpressed genes in GPP patients were enriched in IL\u201017 signalling pathway (<jats:italic>MMP1, MMP3, DEFB4A<\/jats:italic> and <jats:italic>DEFB4B<\/jats:italic>, etc.) and associated with neutrophil infiltration (<jats:italic>MMP1, MMP3, ANXA<\/jats:italic> and <jats:italic>SERPINB,<\/jats:italic> etc.). GPP with <jats:italic>IL36RN<\/jats:italic> mutations evidenced <jats:italic>WNT11<\/jats:italic> upregulation and <jats:italic>IL36RN<\/jats:italic> downregulation in comparison to those GPP without <jats:italic>IL36RN<\/jats:italic> mutations. The expression of IL\u201017A\/IL\u201036 in skin or serum and the origin of IL\u201017A in skin were also investigated. IL\u201017A expression in skin was significantly higher in GPP than PV patients, whereas, there were no differences in skin IL\u201036\u03b1\/IL\u201036\u03b3\/IL\u201036RA or serum IL\u201017A\/IL\u201036\u03b1\/IL\u201036\u03b3 between GPP than PV. Besides, double immunofluorescence staining of MPO\/IL\u201017A or CD3\/IL\u201017A further confirmed that the majority of IL\u201017A in GPP skin was derived from neutrophils, but not T cells. These data emphasized the role of neutrophil\u2010derived IL\u201017A in the pathogenesis of GPP with or withou<jats:italic>t IL36RN<\/jats:italic> mutations. Targeting neutrophil\u2010derived IL\u201017A might be a promising treatment for GPP.<\/jats:p>","DOI":"10.1111\/exd.15026","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T10:37:12Z","timestamp":1707302232000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Increased neutrophil\u2010derived IL\u201017A identified in generalized pustular psoriasis"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6436-0505","authenticated-orcid":false,"given":"Yu","family":"Lan","sequence":"first","affiliation":[{"name":"Department of Dermatology, The Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9708-6060","authenticated-orcid":false,"given":"Xiaoyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Hong Kong\u2010Shenzhen Hospital  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Hong Kong\u2010Shenzhen Hospital  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuechen","family":"Ai","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changxu","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Hong Kong\u2010Shenzhen Hospital  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Eighth Affiliated Hospital Sun Yat\u2010Sen University  Shenzhen China"},{"name":"Department of Dermatology The University of Hong Kong\u2010Shenzhen Hospital  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22169048"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2020.07.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-019-00492-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0519-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-021-00658-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-3388"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14846"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4951"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14523"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1055"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.15250"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.08.056"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.09.035"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2479"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14302"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100123"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15533"},{"key":"e_1_2_9_20_1","first-page":"52","article-title":"RNA sequencing\u2010based longitudinal transcriptomic profiling gives novel insights into the disease mechanism of generalized pustular psoriasis","volume":"11","author":"Wang L","year":"2018","journal-title":"BMC Med Genet"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.10.021"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-09-455022"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.861459"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/1744666X.2018.1438269"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13449"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.16667"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/pde.14737"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.18609"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1186455"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.526"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.817040"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2017.12.024"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14531"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.20809"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.15026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T04:31:47Z","timestamp":1709094707000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.15026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/exd.15026"],"URL":"https:\/\/doi.org\/10.1111\/exd.15026","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e15026"}}