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However, traditional therapies have come under recent scrutiny, such as topical hydroquinone (HQ) has some regulatory restrictions, and high concentration trichloroacetic acid (TCA) peel pose a risk in patients with skin of colour. The objective of our research was to identify, investigate and elucidate the mechanism of action of a novel TCA\u2010 and HQ\u2010free professional\u2010use chemical peel to manage common types of facial hyperpigmentation. Using computational modelling and in\u00a0vitro assays on tyrosinase, we identified proprietary multi\u2010acid synergistic technology (MAST). After a single application on human skin explants, MAST peel was found to be more effective than a commercial HQ peel in inhibiting melanin (histochemical imaging and gene expression). All participants completed the case study (<jats:italic>N<\/jats:italic>\u2009=\u20099) without any adverse events. After administration of the MAST peel by a dermatologist, the scoring and VISIA photography reported improvements in hyperpigmentation, texture and erythema, which could be linked to underlying pathophysiological changes in skin after peeling, visualized by non\u2010invasive optical biopsy of face. Using reflectance confocal microscopy (VivaScope\u00ae) and multiphoton tomography (MPTflex\u2122), we observed reduction in melanin, increase in metabolic activity of keratinocytes, and no signs of inflammatory cells after peeling. Subsequent swabbing of the cheek skin found no microbiota dysbiosis resulting from the chemical peel. The strong efficacy with minimum downtime and no adverse events could be linked to the synergistic action of the ingredients in the novel HQ\u2010 and TCA\u2010free professional peel technology.<\/jats:p>","DOI":"10.1111\/exd.15069","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T13:50:35Z","timestamp":1712152235000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A novel professional\u2010use synergistic peel technology to reduce visible hyperpigmentation on face: Clinical evidence and mechanistic understanding by computational biology and optical biopsy"],"prefix":"10.1111","volume":"33","author":[{"given":"Vinay","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Department of Global Personal Care and Skin Health R&amp;D Colgate\u2010Palmolive Company  Piscataway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc Zachary","family":"Handler","sequence":"additional","affiliation":[{"name":"Dermal Clinical Research Colgate\u2010Palmolive Company  Piscataway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhong","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Global Personal Care and Skin Health R&amp;D Colgate\u2010Palmolive Company  Piscataway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1266-1637","authenticated-orcid":false,"given":"Chloe","family":"Azadegan","sequence":"additional","affiliation":[{"name":"Department of Global Personal Care and Skin Health R&amp;D Colgate\u2010Palmolive Company  Piscataway New Jersey USA"},{"name":"Drexel University College of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pritam K.","family":"Panda","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy Uppsala University  Uppsala Sweden"},{"name":"Nerdalytics  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Georg","family":"Breunig","sequence":"additional","affiliation":[{"name":"JenLab GmbH  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabell","family":"Wenskus","sequence":"additional","affiliation":[{"name":"JenLab GmbH  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Diaz","sequence":"additional","affiliation":[{"name":"Dermal Clinical Research Colgate\u2010Palmolive Company  Piscataway New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"K\u00f6nig","sequence":"additional","affiliation":[{"name":"JenLab GmbH  Berlin Germany"},{"name":"Department of Biophotonics and Laser Technology Saarland University  Saarbrucken Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijwd.2020.09.006"},{"issue":"8","key":"e_1_2_13_3_1","first-page":"13","article-title":"Hyperpigmentation therapy: a review","volume":"7","author":"Desai SR","year":"2014","journal-title":"J Clin Aesthet Dermatol"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2006.01670.x"},{"key":"e_1_2_13_5_1","unstructured":"Food and Drug Administration.FDA works to protect consumers from potentially harmful OTC skin lightening products.2022. 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