{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:49:10Z","timestamp":1775058550453,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T00:00:00Z","timestamp":1719705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The 16th non\u2010collagenous domain (NC16A) of BP180 is the main antigenic target of autoantibodies in bullous pemphigoid (BP) and mucous membrane pemphigoid (MMP). Commercially available assays detect serum autoantibodies against NC16A in the majority of BP (80%\u201390%) and in approximately 50% of MMP patients. However, a standardized test system for detecting antibodies against other regions of BP180 is still lacking. Moreover, anti\u2010BP180 autoantibodies have been found in neurological conditions such as multiple sclerosis and Parkinson disease. This study aimed at identifying primary epitopes recognized by BP autoantibodies on the BP180 ectodomain. Serum samples of 51 BP and 30\u2009MMP patients both without anti\u2010NC16A reactivity were included along with 44 multiple sclerosis and 75 Parkinson disease sera. Four overlapping His\u2010tagged proteins covering the entire BP180 ectodomain (BP180(ec)1\u20134) were cloned, expressed, purified and tested for reactivity by immunoblot. IgG antibodies to BP180(ec)3 were detected in 98% of BP, 77% of MMP and 2% of normal human sera. Only weak reactivity was detected for neurological diseases against BP180(ec)1, BP180(ec)2 and BP180(ec)4, in 3%, 11% and 7% of tested multiple sclerosis sera, respectively. 8% of Parkinson disease sera reacted with BP180(ec)2 and 9% with BP180(ec)4. In conclusion, this study successfully identified epitopes recognized by BP autoantibodies outside the NC16A domain in pemphigoid diseases. These findings contribute to a better understanding of the immune response in BP and MMP with potential implications for a future diagnostic assay for NC16A\u2010negative pemphigoid patients.<\/jats:p>","DOI":"10.1111\/exd.15125","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T06:34:05Z","timestamp":1719815645000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Reactivity against the <scp>BP180<\/scp> ectodomain in patients with bullous pemphigoid, mucous membrane pemphigoid, multiple sclerosis and Parkinson disease"],"prefix":"10.1111","volume":"33","author":[{"given":"Jonathan","family":"Tegtmeyer","sequence":"first","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology (LIED) University of L\u00fcbeck  L\u00fcbeck Germany"},{"name":"Clinic and Polyclinic for Dermatology and Venereology University Medical Center Hamburg\u2010Eppendorf  Hamburg 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