{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T22:46:23Z","timestamp":1792363583871,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acne is a chronic inflammatory skin condition that involves <jats:italic>Cutibacterium acnes<\/jats:italic> (<jats:italic>C. acnes<\/jats:italic>), which is classified into six main phylotypes (IA<jats:sub>1<\/jats:sub>, IA<jats:sub>2<\/jats:sub>, IB, IC, II and III). Acne development is associated with loss of <jats:italic>C. acnes<\/jats:italic> phylotype diversity, characterised by overgrowth of phylotype IA<jats:sub>1<\/jats:sub> relative to other phylotypes. It was also shown that purified extracellular vesicles (EVs) secreted by <jats:italic>C. acnes<\/jats:italic> can induce an acne\u2010like inflammatory response in skin models. We aimed to determine if the inflammatory profile of EVs secreted by <jats:italic>C. acnes<\/jats:italic> phylotype IA<jats:sub>1<\/jats:sub> from an inflammatory acne lesion was different from <jats:italic>C. acnes<\/jats:italic> phylotype IA<jats:sub>1<\/jats:sub> from normal skin, thus playing a direct role in the severity of inflammation. EVs were produced in\u00a0vitro after culture of two clinical strains of <jats:italic>C. acnes<\/jats:italic> phylotype IA<jats:sub>1<\/jats:sub>, T5 from normal human skin and A47 from an inflammatory acne lesion, and then incubated with either human immortalised keratinocytes, HaCaT cells, or skin explants obtained from abdominoplasty. Subsequently, quantitative PCR (qPCR) was performed for human \u03b2\u2010defensin 2 (hBD2), cathelicidin (LL\u201037), interleukin (IL)\u20101\u03b2, IL\u20106, IL\u20108, IL\u201017\u03b1 and IL\u201036\u03b3, and ELISA for IL\u20106, IL\u20108 and IL\u201017\u03b1. We found that EVs produced in\u00a0vitro by <jats:italic>C. acnes<\/jats:italic> derived from inflammatory acne lesions significantly increased the pro\u2010inflammatory cytokines and anti\u2010microbial peptides at both transcriptional and protein levels compared with EVs derived from normal human skin. We show for the first time that <jats:italic>C. acnes<\/jats:italic> EVs from inflammatory acne play a crucial role in acne\u2010associated inflammation in\u00a0vitro and that <jats:italic>C. acnes<\/jats:italic> phylotype IA<jats:sub>1<\/jats:sub> collected from inflammatory acne lesion and normal skin produce different EVs and inflammatory profiles in\u00a0vitro.<\/jats:p>","DOI":"10.1111\/exd.15150","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T07:54:10Z","timestamp":1723103650000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Pro\u2010inflammatory activity of <i>Cutibacterium acnes<\/i> phylotype <i>IA<sub>1<\/sub><\/i> and extracellular vesicles: An in\u00a0vitro study"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7119-4896","authenticated-orcid":false,"given":"Caroline T.","family":"Cheung","sequence":"first","affiliation":[{"name":"Nantes University, INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy (INCIT)  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugo","family":"Lancien","sequence":"additional","affiliation":[{"name":"Nantes University, CHU Nantes Chirurgie Plastique, Reconstructrice et Esth\u00e9tique et Centre de traitement des Br\u00fbl\u00e9s, H\u00f4tel\u2010Dieu  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Corvec","sequence":"additional","affiliation":[{"name":"Nantes University, INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy (INCIT)  Nantes France"},{"name":"CHU Nantes Bacteriology Department Nantes University  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Mengeaud","sequence":"additional","affiliation":[{"name":"Medical Direction, Laboratoires Dermatologiques Ducray, Les Cauquillous  Lavaur France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Mias","sequence":"additional","affiliation":[{"name":"Pierre Fabre Dermo\u2010Cosm\u00e9tique et Personal Care  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00eblle","family":"V\u00e9ziers","sequence":"additional","affiliation":[{"name":"Nantes University, Oniris, CHU Nantes, INSERM, Regenerative Medicine and Skeleton, RMeS  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Khammari","sequence":"additional","affiliation":[{"name":"Nantes University, INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy (INCIT)  Nantes France"},{"name":"Department of Dermatology Nantes University, CHU Nantes, INSERM  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5574-5825","authenticated-orcid":false,"given":"Brigitte","family":"Dr\u00e9no","sequence":"additional","affiliation":[{"name":"Nantes University, INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy (INCIT)  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/S40257-020-00531-1\/FIGURES\/2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14820"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02460-14"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANNDER.2018.11.002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.r06129-11"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104199"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.21"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.15795"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012277"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2847"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/EXD.14467"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioflm.2021.100063"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.01.007"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/PRCA.201600040"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCID.S363537"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41598-023-43354-W"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23105797"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/NMETH.2019"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.6026\/97320630015369"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41579-018-0112-2"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41579-023-00875-5"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00433-20"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3480"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/JDV.13190"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2015.2713"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/JDV.18793"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/JDV.18792"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S374692"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23705.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.04.001"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2018.09.005"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-25436-3"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/MICROORGANISMS9020303"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41467-023-43833-8"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23095065"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167237.s003\u00ef"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2023.05.029"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.841"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.15150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T15:48:24Z","timestamp":1746632904000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.15150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/exd.15150"],"URL":"https:\/\/doi.org\/10.1111\/exd.15150","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"2024-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e15150"}}