{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T22:24:23Z","timestamp":1776464663062,"version":"3.51.2"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diagnosis of S\u00e9zary syndrome (SS) relies on the identification of blood S\u00e9zary cells (SC) by different markers via flow cytometry. Treatment of SS is challenging since its pathogenesis is characterized by cell death resistance rather than hyperproliferation. In this study, we establish an integrated approach that considers both the expression of SC markers and sensitivity to cell death both spontaneously and upon in\u00a0vitro treatment. Peripheral blood mononuclear cells were isolated from 20 SS patients and analysed for the SC markers CD7 and CD26 loss as well as CD158k and PD1 gain. The cells were then treated with different established and experimental therapies in\u00a0vitro and cell death was measured. Spontaneous and therapeutically induced cell death were measured and correlated to cellular marker profiles. In the marker\u2010positive cells, spontaneous cell death sensitivity was reduced. Different treatments in\u00a0vitro managed to specifically induce cell death in the putative CTCL cell populations. Interestingly, a repeated analysis after 3 months of treatment revealed the CTCL cell death sensitivity to be restored by therapy. We propose this novel integrated approach comprising the evaluation of SC marker expression and analysis of cell death sensitivity upon treatment that can also enable a better therapy stratification.<\/jats:p>","DOI":"10.1111\/exd.15171","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T05:21:21Z","timestamp":1725254481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of S\u00e9zary cell marker expression and cell death behaviour upon in\u00a0vitro treatment by flow cytometry in S\u00e9zary syndrome patients"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6456-2681","authenticated-orcid":false,"given":"S.","family":"Melchers","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology University Medical Center Mannheim\/University of Heidelberg  Mannheim Germany"},{"name":"Skin Cancer Unit German Cancer Research Center (DKFZ)  Heidelberg Germany"},{"name":"Section of Clinical and Experimental Dermatology, Medical Faculty Mannheim University of Heidelberg  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2113-6287","authenticated-orcid":false,"given":"M.","family":"Roemer","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Laboratory Medicine University Medical Center Mannheim, Ruprecht\u2010Karls\u2010University of Heidelberg  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. D.","family":"Albrecht","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology University Medical Center Mannheim\/University of Heidelberg  Mannheim Germany"},{"name":"Skin Cancer Unit German Cancer Research Center (DKFZ)  Heidelberg Germany"},{"name":"Section of Clinical and Experimental Dermatology, Medical Faculty Mannheim University of Heidelberg  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Assaf","sequence":"additional","affiliation":[{"name":"Department of Dermatology HELIOS Klinik Krefeld  Krefeld Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"von Gugelberg","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5478-8735","authenticated-orcid":false,"given":"E.","family":"Guenova","sequence":"additional","affiliation":[{"name":"Department of Dermatology Lausanne University Hospital  Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u2010D.","family":"Klemke","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Municipal Medical Center Karlsruhe Teaching Hospital of the University of Freiburg  Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. K. C.","family":"Moritz","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Halle  Halle Germany"},{"name":"Department of Dermatology, Venerology and Allergology Freie Universit\u00e4t Berlin and Humboldt\u2010Universit\u00e4t zu Berlin, University Medical Centre Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Schlaak","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2683-6028","authenticated-orcid":false,"given":"R.","family":"Stadler","sequence":"additional","affiliation":[{"name":"Department of Dermatology Johannes\u2010Wesling\u2010Clinic Minden and University of Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7398-4261","authenticated-orcid":false,"given":"U.","family":"Wehkamp","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Kiel  Kiel Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6293-2554","authenticated-orcid":false,"given":"M.","family":"Wobser","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Wurzburg  Wurzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Albrecht","sequence":"additional","affiliation":[{"name":"Department of Pathology Ruprechts\u2010Karls\u2010University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Goerdt","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology University Medical Center Mannheim\/University of Heidelberg  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Laboratory Medicine University Medical Center Mannheim, Ruprecht\u2010Karls\u2010University of Heidelberg  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0220-0551","authenticated-orcid":false,"given":"J. P.","family":"Nicolay","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology University Medical Center Mannheim\/University of Heidelberg  Mannheim Germany"},{"name":"Skin Cancer Unit German Cancer Research Center (DKFZ)  Heidelberg Germany"},{"name":"Section of Clinical and Experimental Dermatology, Medical Faculty Mannheim University of Heidelberg  Mannheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-11-881268"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-022-01620-2"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-03-055749"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08739.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0303-6987.2006.00494.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.01.038"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP1NA3YCHCDEIG"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2018.01.076"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04014.x"},{"issue":"1","key":"e_1_2_11_11_1","article-title":"Blockade of programmed cell death protein 1 (PD\u20101) in Sezary syndrome reduces Th2 phenotype of non\u2010tumoral T lymphocytes but may enhance tumor proliferation","volume":"9","author":"Saulite I","year":"2020","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.21053"},{"issue":"3","key":"e_1_2_11_13_1","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1111\/ddg.12900","article-title":"Sezary syndrome: old enigmas, new targets","volume":"14","author":"Nicolay JP","year":"2016","journal-title":"J Dtsch Dermatol Ges"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-09-598995"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.286"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-4631"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2001.tb03710.x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23657.x"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-694117"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022018669"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60420-1"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2017.02.027"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/1074-5521(95)90120-5"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1311695"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.5301\/uro.5000195"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0000000000000729"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPV2D1DDSGJDBW"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00163961"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01895-3"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2002.118538"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-07-588236"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.5.1388"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.356"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21962"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.21063"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.22070"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2010.200"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.19669"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021005147"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2021.2191"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2015.12.018"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.2340\/actadv.v102.1062"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.21018"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.21922.1"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021012286"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.120"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019001545"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43168"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312933"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCO.0000000000000243"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.15171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T08:43:33Z","timestamp":1738053813000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.15171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":49,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/exd.15171"],"URL":"https:\/\/doi.org\/10.1111\/exd.15171","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2024-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e15171"}}