{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:59:40Z","timestamp":1770339580454,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The pemphigoid disease epidermolysis bullosa acquisita (EBA) is an autoimmune blistering skin disease characterized by autoantibodies against type VII collagen (COL7), immune cell infiltrates at the dermal\u2013epidermal junction and subepidermal blistering. Proteases, particularly granzyme B (GzmB), have been established as therapeutic targets for the treatment of EBA and other pemphigoid diseases. We investigated the impact of the novel GzmB inhibitor SNT\u20106935 on anti\u2010COL7 IgG\u2010induced subepidermal blistering in a well\u2010established EBA ex\u00a0vivo model. Our findings demonstrate that pharmacological targeting of GzmB with its selective inhibitor SNT\u20106935 significantly reduced autoantibody\u2010induced dermal\u2013epidermal separation in human skin cryosections. Interestingly, treatment of skin cryosections with recombinant human GzmB alone did not cause dermal\u2013epidermal separation, suggesting that additional mechanisms alongside GzmB are required for tissue damage in EBA. In conclusion, our study highlights the significant contribution of GzmB to the pathogenesis of EBA and supports the notion of GzmB as a therapeutic target in EBA and other pemphigoid diseases.<\/jats:p>","DOI":"10.1111\/exd.15172","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T05:09:37Z","timestamp":1725253777000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Granzyme B inhibition reduces autoantibody\u2010induced dermal\u2013epidermal separation in an ex\u00a0vivo model of epidermolysis bullosa acquisita"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7965-8045","authenticated-orcid":false,"given":"Shirin","family":"Emtenani","sequence":"first","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology (LIED) University of L\u00fcbeck  L\u00fcbeck 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