{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:40:04Z","timestamp":1789386004572,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In order to explore the huge impact of impaired immnue homeostasis on the occurrence and development of cutaneous squamous cell carcinoma (cSCC), and investigate characterization of the cellular components and their changes which is crucial to understanding the pathologic process of HPV\u2010induced cSCC, we diagnosed and followed up on a very rare HPV\u2010induced cSCC patient who progressed at a very fast rate and transferred to death quickly. We performed single\u2010cell RNA sequencing (scRNA\u2010seq) of 11\u2009379 cells from the skin tissues of this patient with four different skin statuses after HPV infection. Immunofluorescence experiments were used for validation. scRNA\u2010seq identified that CD52\n                    <jats:sup>+<\/jats:sup>\n                    HLA\u2010DOA\n                    <jats:sup>\u2010<\/jats:sup>\n                    macrophages only existed in paracancerous cutaneous squamous cell carcinoma (pc\u2010cSCC) and cSCC tissue. Besides, immune cells including CD8\n                    <jats:sup>+<\/jats:sup>\n                    exhausted T cells and CD4\n                    <jats:sup>+<\/jats:sup>\n                    regulatory T cells as well as matrix cells like MMP1\n                    <jats:sup>+<\/jats:sup>\n                    , and MMP11\n                    <jats:sup>+<\/jats:sup>\n                    fibroblasts were gradually increased. Meanwhile, COMP\n                    <jats:sup>+<\/jats:sup>\n                    ASPN\n                    <jats:sup>+<\/jats:sup>\n                    fibroblasts gradually decreased. Cell interaction analysis revealed enhancement in interactions between monocytes\/macrophages, fibroblasts and tumour\u2010specific keratinocytes. scRNA\u2010seq was performed in HPV\u2010induced cSCC for the first time, to explore the correlation between infection and tumour. It is the first time to study the development of tumours from different stages of infection in HPV\u2010induced cSCC. In this study, the tumour itself and the tumour microenvironment were both analysed and explored. And it was validated in clinical samples from different patients. Our findings reveal the dynamic immnue homeostasis from normal skin\u00a0to cSCC tissue, this alteration might drive HPV\u2010induced cSCC.\n                  <\/jats:p>","DOI":"10.1111\/exd.15178","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T09:12:29Z","timestamp":1728551549000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single cell transcriptomics reveals dysregulated immnue homeostasis in different stages in\n                    <scp>HPV<\/scp>\n                    \u2010induced cutaneous squamous cell carcinoma"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5740-7344","authenticated-orcid":false,"given":"Liqing","family":"Ding","sequence":"first","affiliation":[{"name":"The Department of Rheumatology and Immunology Xiangya Hospital of Central South University  Changsha Hunan China"},{"name":"Provincial Clinical Research Center for Rheumatic and Immunologic Diseases Xiangya Hospital  Changsha Hunan China"},{"name":"National Clinical Research Center for Geriatric Disorders Xiangya Hospital  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanyuan","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Xiangya Hospital Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology  Changsha Hunan China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Central South University  Changsha Hunan China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Xiangya Hospital, Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunlin","family":"Qu","sequence":"additional","affiliation":[{"name":"Pathophysiology Department, Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Hengyang Medical School University of South China  Hengyang Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongjie","family":"Li","sequence":"additional","affiliation":[{"name":"Provincial Clinical Research Center for Rheumatic and Immunologic Diseases Xiangya Hospital  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Yao","sequence":"additional","affiliation":[{"name":"Tencent AI Lab  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Tencent AI Lab  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglin","family":"Zhu","sequence":"additional","affiliation":[{"name":"The Department of Rheumatology and Immunology Xiangya Hospital of Central South University  Changsha Hunan China"},{"name":"Provincial Clinical Research Center for Rheumatic and Immunologic Diseases Xiangya Hospital  Changsha Hunan China"},{"name":"National Clinical Research Center for Geriatric Disorders Xiangya Hospital  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology  Changsha Hunan China"},{"name":"Hunan Engineering Research Center of Skin Health and Disease Central South University  Changsha Hunan China"},{"name":"Hunan Key Laboratory of Skin Cancer and Psoriasis Xiangya Hospital, Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082956"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30925-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.4046"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.08.059"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2012.02558.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.05.039"},{"key":"e_1_2_9_8_1","first-page":"1346","article-title":"Predicting cancerization of condyloma 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