{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:53:56Z","timestamp":1770900836297,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073438"],"award-info":[{"award-number":["82073438"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017668","name":"Anhui Provincial Key Research and Development Plan","doi-asserted-by":"publisher","award":["2022e07020041"],"award-info":[{"award-number":["2022e07020041"]}],"id":[{"id":"10.13039\/501100017668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Pemphigus vulgaris (PV) stands as a rare autoimmune bullous disease, while the precise underlying mechanism remains incompletely elucidated. High\u2010throughput proteomic methodologies, such as LC\u2010MS\/MS, have facilitated the quantification and characterisation of proteomes from clinical skin samples, enhancing our comprehension of PV pathogenesis. The objective of this study is to elucidate the signalling mechanisms underlying PV through proteomic analysis. Proteins and cell suspension were extracted from skin biopsies obtained from both PV patients and healthy volunteers and subsequently analysed using LC\u2010MS\/MS and scRNA\u2010seq. Cultured keratinocytes were treated with PV serum, followed by an assessment of protein expression levels using immunofluorescence and western blotting. A total of 880, 605, and 586 differentially expressed proteins (DEPs) were identified between the lesion vs. control, non\u2010lesion vs. control, and lesion vs. non\u2010lesion groups, respectively. The oxidative phosphorylation (OXPHOS) pathway showed activation in PV. Keratinocytes are the major cell population in the epidermis and highly expressed ATP5PF, ATP6V1G1, COX6B1, COX6A1, and NDUFA9. In the cellular model, there was a notable increase in the expression levels of OXPHOS\u2010related proteins (V\u2010ATP5A, III\u2010UQCRC2, II\u2010SDHB, I\u2010NDUFB8), along with STAT1, p\u2010STAT1, and p\u2010JAK1. Furthermore, both the OXPHOS inhibitor metformin and the JAK1 inhibitor tofacitinib demonstrated therapeutic effects on PV serum\u2010induced cell separation, attenuating cell detachment. Metformin notably reduced the expression of V\u2010ATP5A, III\u2010UQCRC2, II\u2010SDHB, I\u2010NDUFB8, p\u2010STAT1, p\u2010JAK1, whereas tofacitinib decreased the expression of p\u2010STAT1 and p\u2010JAK1, with minimal impact on the expression of V\u2010ATP5A, III\u2010UQCRC2, II\u2010SDHB, and I\u2010NDUFB8. Our results indicate a potential involvement of the OXPHOS and JAK\u2010STAT1 pathways in the pathogenesis of PV.<\/jats:p>","DOI":"10.1111\/exd.15184","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T07:15:46Z","timestamp":1728285346000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Proteomic Analysis Reveals Oxidative Phosphorylation and\n                    <scp>JAK<\/scp>\n                    \u2010\n                    <scp>STAT<\/scp>\n                    Pathways Mediated Pathogenesis of Pemphigus Vulgaris"],"prefix":"10.1111","volume":"33","author":[{"given":"Yuqi","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital, Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Dermatology Anhui Medical University  Hefei Anhui China"},{"name":"Key Laboratory of Dermatology (Anhui Medical University) Ministry of Education  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital, Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Dermatology Anhui Medical University  Hefei Anhui China"},{"name":"Key Laboratory of Dermatology (Anhui Medical University) Ministry of Education  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CaiHong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital, Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Dermatology Anhui Medical University  Hefei Anhui China"},{"name":"Key Laboratory of Dermatology (Anhui Medical University) Ministry of Education  Hefei Anhui 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