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Therefore, this study aimed to clarify the mechanism of IL\u201033 action in decubitus ulcer formation. IL\u201033 knockout (KO), soluble stimulation\u20102 (ST2) transgenic, and wild\u2010type (WT) mice were used to construct an ischemia\u2013reperfusion (I\/R) injury as a decubitus model. The ulcer area was significantly reduced in IL\u201033 KO mice compared to WT mice but was not reduced in ST2 transgenic mice. Anti\u2010IL\u201033 receptor (transmembrane ST2) antibodies effectively prevented ulcer formation; however, an anti\u2010IL\u201033 neutralising antibody was ineffective. The number of infiltrating macrophages was higher, while that of neutrophils and mast cells was lower in IL\u201033 KO mice than in WT mice. The number of M2 macrophages increased in IL\u201033 KO mice. Characterisation of gene expression levels revealed significantly reduced interleukin\u20101 beta (IL\u20101\u03b2) and increased C\u2010C motif chemokine ligand 17 expression in IL\u201033 KO mice. Macrophages isolated from ulcers in WT or IL\u201033 KO mice stimulated with exogenous IL\u201033 produced comparable amounts of IL\u20101\u03b2. In conclusion, our study indicates that IL\u201033 is released in response to I\/R injury in the skin, contributing to inflammatory macrophage and mast cell infiltration and stimulation, resulting in IL\u20101\u03b2 production and the massive infiltration of effector cells, including neutrophils, which finally induces decubitus ulcer formation. These results suggest that suppressing IL\u201033 expression could be beneficial for treating early\u2010phase decubitus ulcers.<\/jats:p>","DOI":"10.1111\/exd.70014","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T12:14:04Z","timestamp":1731932044000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interleukin\u201033 Deficiency Protects the Skin From Ulcer Formation in an Ischemia\u2013Reperfusion\u2010Induced Decubitus Mouse Model"],"prefix":"10.1111","volume":"33","author":[{"given":"Meijuan","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Dermatology Jichi Medical University  Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1086-1803","authenticated-orcid":false,"given":"Mayumi","family":"Komine","sequence":"additional","affiliation":[{"name":"Department of Dermatology Jichi Medical 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