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To unravel the role of PAF, we focused on lysophosphatidylcholine acyltransferase 2 (LPCAT2 also called LPLAT9), a biosynthetic enzyme involved in PAF production, and investigated the role of PAF in allergic contact dermatitis (ACD) and irritant contact dermatitis (ICD). We measured the amount of PAF in the skin and investigated the ear swelling responses and leukocyte infiltration into the skin following the application of 2,4,6\u2010trinitro\u20101\u2010chlorobenzene (TNCB) or croton oil in wild\u2010type (WT) and <jats:italic>LPCAT2<\/jats:italic> knockout (<jats:italic>LPCAT2<\/jats:italic>\u2010KO) mice. The amount of PAF was increased in the skin of WT mice after TNCB or croton oil application but not detected in <jats:italic>LPCAT2<\/jats:italic>\u2010KO mice. The ear swelling response was decreased in <jats:italic>LPCAT2<\/jats:italic>\u2010KO mice compared with that in WT mice. In the ACD model, the numbers of lymphocytes, eosinophils, macrophages, mast cells and neutrophils were smaller in <jats:italic>LPCAT2<\/jats:italic>\u2010KO mice than in WT mice. In the ICD model, the ear swelling response was also decreased in <jats:italic>LPCAT2<\/jats:italic>\u2010KO mice compared with that in WT mice. When double staining of each inflammatory cell type and LPCAT2 was performed in ACD tissue, marked co\u2010staining of the eosinophil marker and LPCAT2 was observed. In addition, LPCAT2 expression was observed in the epidermis. These results indicate that PAF is involved in the infiltration of several cell types into the sites of allergic and non\u2010allergic skin inflammation. Furthermore, eosinophils and keratinocytes are primarily responsible for PAF production in skin inflammation.<\/jats:p>","DOI":"10.1111\/exd.70015","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T14:23:48Z","timestamp":1731075828000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lysophosphatidylcholine Acyltransferase 2 Contributes to Increased Allergic and Irritant Inflammation in Mice"],"prefix":"10.1111","volume":"33","author":[{"given":"Midori","family":"Kawasaki\u2010Nagano","sequence":"first","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9379-9584","authenticated-orcid":false,"given":"Risa","family":"Tamagawa\u2010Mineoka","sequence":"additional","affiliation":[{"name":"Department 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