{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T03:31:17Z","timestamp":1786591877094,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012247","name":"Program of Shanghai Academic Research Leader","doi-asserted-by":"publisher","award":["23XD1400400"],"award-info":[{"award-number":["23XD1400400"]}],"id":[{"id":"10.13039\/501100012247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82304016"],"award-info":[{"award-number":["82304016"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273531"],"award-info":[{"award-number":["82273531"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82330098"],"award-info":[{"award-number":["82330098"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373489"],"award-info":[{"award-number":["82373489"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Atopic dermatitis (AD) patients usually have elevated serum IgE that is thought inducing inflammation upon binding to allergen. However, the role of IgE\u2010producing B cells and other isotypes of immunoglobulin, such as IgG, in AD are not clear and rarely explored. This study aimed to investigate the role of IgE\u2010producing B cells and other isotypes of immunoglobulin, particularly IgG, in skin lesion of AD. BCR repertoires were analysed using mRNA prepared from skin lesions and peripheral blood mononuclear cells (PBMCs) from AD patients and non\u2010allergic healthy subjects. Single\u2010cell RNA sequencing data of AD lesions from published literature were extracted to analyse the function of IgG. BCR repertoires from skin lesion and PBMCs clustered distinctly, and PBMCs showed higher interindividual similarity compared to those from the skin. The proportions of IGHM, IGHD, IGHA, IGHG and IGHE varied among skin lesion and PBMCs of AD patients and healthy individuals, and IGHG was significantly increased in AD lesion. IGHG showed biased VH usage, with dominance of V1\u201058, V1\u20108, V3\u201013 and V3\u201073. The much higher hyperexpanded clonality and lower diversity of IGHG repertoire in skin than those of the PBMCs, suggested the clonal expansion of IgG\n                    <jats:sup>+<\/jats:sup>\n                    B cells in the skin. Pathways related with IgG activation were enriched in AD skin, and macrophages may be activated by IgG and promote skin inflammation. In conclusion, skin is not the main production site for IgE in AD. IgG may involve in promoting Th2 inflammation in AD skin through macrophages.\n                  <\/jats:p>","DOI":"10.1111\/exd.70058","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T05:20:41Z","timestamp":1738819241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["IgG Signalling Involves in Skin Inflammation of Atopic Dermatitis"],"prefix":"10.1111","volume":"34","author":[{"given":"Jing","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5416-1743","authenticated-orcid":false,"given":"Xingyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Allergy and Rheumatology, Hospital for Skin Diseases, Institute of Dermatology, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs Chinese Academy of Medical Sciences and Peking Union Medical College  Nanjing P.\u00a0R.\u00a0China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Huashan Hospital Fudan University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronghui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huibin","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liya","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangshang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoyin","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Allergy and Rheumatology, Hospital for Skin Diseases, Institute of Dermatology, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs Chinese Academy of Medical Sciences and Peking Union Medical College  Nanjing P.\u00a0R.\u00a0China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1186-8609","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai Institute of Dermatology, National Clinical Research Center for Aging and Medicine, Huashan Hospital Fudan\u00a0University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31286-1"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.08.027"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.02.042"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15601"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132173"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202000451"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15173"},{"issue":"10","key":"e_1_2_13_9_1","first-page":"1067","article-title":"Cross\u2010Reacting IgE and IgG Antibodies to Pityrosporum Ovale Mannan and Other Yeasts in Atopic Dermatitis","volume":"54","author":"Lintu P.","year":"1999","journal-title":"Allergy (Oslo, Norway)"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056002180.x"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/2050640614544314"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024967426025"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2001.00745.x"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2010.549157"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16153"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.2002.01029.x"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2000.03534.x"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.880412"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23126867"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.13907"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.14688"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00786-1"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.603050"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba6500"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.07.717"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.01.006"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.2000.15.11.x"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12054"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.10.5602"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15413"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.07.014"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400692"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115593"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-011-9541-7"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.3.887"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2024.07.017"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/71908"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2010.07.004"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.824008"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2021.07.187"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70058","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.70058","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T03:25:50Z","timestamp":1786591550000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.70058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/exd.70058"],"URL":"https:\/\/doi.org\/10.1111\/exd.70058","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70058"}}