{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T03:31:20Z","timestamp":1786591880020,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FOR2372"],"award-info":[{"award-number":["FOR2372"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Invasive growth and metastatic dissemination represent the primary cause of death in cancer patients. In order to successfully detach from the primary tumour and establish metastases in distant tissues, cancer cells need to dynamically rewire their cell adhesion machinery. Here we revisit the potential association of MCAM, a member of the immunoglobulin superfamily that was initially identified as a melanoma antigen, with disease progression. Using immunohistochemical stainings and bioinformatic analyses of published datasets, we find abundant MCAM expression both in primary and metastatic human melanomas. In additional bioinformatic analyses, we show that MCAM is highly expressed in foetal melanocytes and subsequently downregulated during melanocyte maturation. Bioinformatic inference of cellular communication networks reveals that melanoma cells with high MCAM expression more actively engage in signalling crosstalk with endothelial cells. Experimental investigations demonstrate that disruption of MCAM in melanoma cells inhibits their migration on endothelial cell surfaces in\u00a0vitro and decreases their ability to develop spontaneous lung metastases in\u00a0vivo. Taken together, our results could not confirm the notion that MCAM expression represents a useful biomarker for disease progression but provide evidence that MCAM expression might represent part of a reactivated embryonal transcriptional program that facilitates melanoma\u2013endothelial cell interactions during metastatic progression.<\/jats:p>","DOI":"10.1111\/exd.70059","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T04:39:51Z","timestamp":1739421591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>MCAM<\/scp>\n                    Expression Facilitates Melanoma\u2013Endothelial Interactions and Promotes Metastatic Disease Progression"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6954-0633","authenticated-orcid":false,"given":"Andreas Dominik","family":"Braun","sequence":"first","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Mengoni","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"T\u00fcting","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Gaffal","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"},{"name":"Department of Dermatology, Allergy, and Venereology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.03.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-018-0038-z"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0080-4"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV119.007759"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2012.11.049"},{"key":"e_1_2_10_7_1","first-page":"841","article-title":"Discrimination Between Benign and Malignant Cells of Melanocytic Lineage by Two Novel Antigens, a Glycoprotein With a Molecular Weight of 113\u2009000 and a Protein With a Molecular Weight of 76\u2009000","volume":"47","author":"Lehmann J. M.","year":"1987","journal-title":"Cancer Research"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.24.9891"},{"key":"e_1_2_10_9_1","first-page":"2514","article-title":"Isolation and Functional Characterization of the A32 Melanoma\u2010Associated Antigen","volume":"54","author":"Shih I.\u2010M.","year":"1994","journal-title":"Cancer Research"},{"key":"e_1_2_10_10_1","first-page":"2295","article-title":"Expression of MCAM\/MUC18 by Human Melanoma Cells Leads to Increased Tumor Growth and Metastasis","volume":"57","author":"Xie S.","year":"1997","journal-title":"Cancer Research"},{"key":"e_1_2_10_11_1","first-page":"3835","article-title":"Melanoma Cell\u2010Cell Interactions Are Mediated Through Heterophilic Mel\u2010CAM\/Ligand Adhesion","volume":"57","author":"Shih I.\u2010M.","year":"1997","journal-title":"Cancer Research"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13111"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40896-w"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.09.016"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.044"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0095"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010021\u201000740\u20108"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010017\u20101382\u20100"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2018.11.005"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0947-7"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05242-7"},{"key":"e_1_2_10_22_1","doi-asserted-by":"crossref","unstructured":"S.Jin M. 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