{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T04:26:50Z","timestamp":1786595210202,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Atopic dermatitis (AD) is a common skin disease. Although AD pathogenesis has been widely researched, inhibitory mechanisms in AD are still unclear. Sialic acid\u2010binding immunoglobulin\u2010like lectins (Siglecs) are a family of receptors recognising sialic acids; most Siglecs work as inhibitory receptors. Among Siglecs, Siglec\u2010E is expressed on dendritic cells (DCs) and eosinophils, important immune cells in AD. Although Siglec\u2010E inhibits Type 1 inflammatory diseases, how it influences AD is unknown. Thus, we investigated the role of Siglec\u2010E in AD mouse model by using\n                    <jats:italic>Siglec\u2010E<\/jats:italic>\n                    knockout (KO) mice. We demonstrated that Siglec\u2010E attenuated AD\u2010like inflammation of mice caused by topical application of MC903 on ear skin (MC903\u2010induced AD). To reveal the role of Siglec\u2010E in MC903\u2010induced AD, we focused on Siglec\u2010E on DCs and eosinophils. We first showed that Sigle\u2010E was expressed on cutaneous DCs and migratory DCs of draining lymph nodes. Moreover, OX40L expression on cutaneous DCs was reduced in the presence of Siglec\u2010E. In\u00a0vitro experiments using cultured spleen DCs (SpDCs), highly expressing Siglec\u2010E, revealed that IL\u201033 was involved in the induction of Siglec\u2010E and confirmed that Siglec\u2010E inhibited OX40L expression on SpDCs induced by IL\u201033. Moreover, CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell\u2013SpDC coculture revealed that Siglec\u2010E inhibited Th2 polarisation under IL\u201033 stimulation. We finally revealed that Siglec\u2010E was expressed on eosinophils and reduced the eosinophils infiltration to the MC903\u2010treated ear skin with the suppression of CD49d, a necessary integrin for eosinophil migration to skin tissue [1], expression on eosinophils. These findings elucidated the inhibitory role of Siglec\u2010E in MC903\u2010induced AD.\n                  <\/jats:p>","DOI":"10.1111\/exd.70064","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T04:04:37Z","timestamp":1739937877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Role of Siglec\u2010E in\n                    <scp>MC903<\/scp>\n                    \u2010Induced Atopic Dermatitis"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0478-7902","authenticated-orcid":false,"given":"Mari","family":"Nakanishi","sequence":"first","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto 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