{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T13:16:18Z","timestamp":1780060578768,"version":"3.54.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ikaros, which is encoded by the Ikaros family zinc finger 1 (<jats:italic>IKZF1<\/jats:italic>) gene, is a zinc finger transcription factor. We have previously generated K5\u2010<jats:italic>Ikzf1<\/jats:italic>\u2010EGFP transgenic mice (<jats:italic>Ikzf1<\/jats:italic> Tg) by introducing the IKZF1 isoform into epithelial cells expressing keratin 5, which develop patchy alopecia. However, there has been no detailed in\u00a0vivo investigation of the function of <jats:italic>IKZF1<\/jats:italic> in alopecia or of Ikaros expression in hair follicles of alopecia patients. Our aim was to investigate whether <jats:italic>IKZF1<\/jats:italic> overexpression is involved in the pathogenesis of alopecia areata (AA) using <jats:italic>Ikzf1<\/jats:italic> Tg and to examine Ikaros expression in human scalp skin. We grossly and histologically examined the alopecic lesions of <jats:italic>Ikzf1<\/jats:italic> Tg and the skin of wild\u2010type (WT) mice and the associated mRNA expression of inflammatory mediators. We also examined Ikaros' expression in human scalp skin. Grossly and histologically, we found that the <jats:italic>Ikzf1<\/jats:italic> Tg developed AA\u2010like lesions. Immunohistochemically, the hair follicles of the <jats:italic>Ikzf1<\/jats:italic> Tg expressed high levels of the NKG2D ligand H60 and contained infiltrating CD8<jats:sup>+<\/jats:sup>NKG2D<jats:sup>+<\/jats:sup> T cells. Interleukin 15, tumour necrosis factor\u2010\u03b1, CXC chemokine ligand (Cxcl)1, Cxcl10, Cxcl11, signal transducer and activator of transcription (STAT)1, STAT3, Janus kinase (JAK)1 and JAK3 mRNA expression were significantly higher in the alopecic lesions of the <jats:italic>Ikzf1<\/jats:italic> Tg than in the WT mice. <jats:italic>Ikzf1<\/jats:italic> Tg given corticosteroid injections exhibited hair regrowth. Immunohistochemical analysis of scalp hair follicles showed that Ikaros was more highly expressed in AA patients than in non\u2010AA controls. Our study suggests that <jats:italic>IKZF1<\/jats:italic> and Ikaros are involved in the pathogenesis of AA.<\/jats:p>","DOI":"10.1111\/exd.70074","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T12:12:50Z","timestamp":1741695170000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>IKZF1<\/i> and Ikaros Overexpression Results in Alopecia Areata\u2010Like Phenotype in Mice"],"prefix":"10.1111","volume":"34","author":[{"given":"Yukiyasu","family":"Arakawa","sequence":"first","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9379-9584","authenticated-orcid":false,"given":"Risa","family":"Tamagawa\u2010Mineoka","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayumi","family":"Ueta","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Nishigaki","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norito","family":"Katoh","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical Science Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2024.03.018"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13308"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13753"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1103442"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70195-7"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2006.01676.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3645"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09114"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.11.001"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100657"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12382416"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30997-3"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2021.04.009"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8019.2011.01419.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14129"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.12970"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501798"},{"issue":"1","key":"e_1_2_11_19_1","first-page":"40","article-title":"Differential Expression of CXCL1, CXCL9, CXCL10 and CXCL12 Chemokines in Alopecia Areata","volume":"10","author":"Zainodini N.","year":"2013","journal-title":"Iranian Journal of Immunology"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1439790"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1993.tb11842.x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(84)70152-6"},{"issue":"2","key":"e_1_2_11_23_1","first-page":"208","article-title":"T Cell Subpopulations and Their Functions In\u00a0Vitro. 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