{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T03:32:01Z","timestamp":1773199921127,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Reactive oxygen species (ROS) are involved in the pathogenesis of generalised pustular psoriasis (GPP), but this involvement has not been fully elucidated. We performed the diacron\u2010reactive oxygen metabolite (d\u2010ROM) test and the biological antioxidant potential (BAP) test on sera from nine patients with active GPP who were hospitalised and treated at our hospital, including three patients with pustular psoriasis of pregnancy (PPP). The serum d\u2010ROM and BAP levels were evaluated before treatment and at 1\u2009month of treatment. We also performed immunostaining of 4\u2010hydroxy\u20102\u2010nonenal (4\u2010HNE) in skin tissues. In the GPP patients, the average d\u2010ROM levels were significantly reduced at 1\u2009month of treatment (reduced to 343.0\u2009\u00b1\u200982.1\u2009U.Carr from 423.2\u2009\u00b1\u200995.0\u2009U.Carr, <jats:italic>p<\/jats:italic>\u2009=\u20090.005). The Generalised Pustular Psoriasis Area and Severity Index (GPPASI) score correlated with d\u2010ROM levels (<jats:italic>r<\/jats:italic>\u2009=\u20090.57, <jats:italic>p<\/jats:italic>\u2009=\u20090.10), suggesting that those levels reflect the disease severity. In normal pregnancy, d\u2010ROM values are known to increase from mid\u2010term to late\u2010term. The d\u2010ROM values increased when GPP worsened in the case of PPP. Immunohistochemical staining of 4\u2010HNE was positive for subcorneal pustules, neutrophils, and for the cytoplasm of epidermal keratinocytes, especially in upper epidermal layers. Our findings indicate that 4\u2010HNE may play an important role in GPP and PPP.<\/jats:p>","DOI":"10.1111\/exd.70076","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T22:58:07Z","timestamp":1741733887000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Oxidative Stress and Generalised Pustular Psoriasis: Report of d\u2010<scp>ROM<\/scp> Measurements in Nine Cases Including Three of Pustular Psoriasis of Pregnancy"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4555-8297","authenticated-orcid":false,"given":"Chisato","family":"Tawada","sequence":"first","affiliation":[{"name":"Department of Dermatology Gifu University Graduate School of Medicine  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5229-1669","authenticated-orcid":false,"given":"Yoko","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Gifu University Graduate School of Medicine  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Department of Dermatology Gifu University Graduate School of Medicine  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Zang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Gifu University Graduate School of Medicine  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayoko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Dermatology Gifu University Graduate School of Medicine  Gifu 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