{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:18:56Z","timestamp":1758759536673,"version":"3.44.0"},"reference-count":8,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["318346496"],"award-info":[{"award-number":["318346496"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Treatment against leishmaniasis is associated with severe side effects, high costs, and parasitic resistance. Preclinical models such as humanised mice would aid therapeutic improvement or the development of a vaccine. We developed a model in which human skin transplants on immunodeficient mice are infected with <jats:styled-content style=\"fixed-case\"><jats:italic>Leishmania major<\/jats:italic><\/jats:styled-content>. Parasite inoculation of the skin transplant led to a robust infection with increasing numbers of parasites in the skin and visceral organs. In addition, intraperitoneally co\u2010administered allogeneic peripheral blood mononuclear cells (PBMCs) were strongly recruited to skin lesions, with \u2265 65% of the cells being positive for anti\u2010human CD45; we identified ~20% CD4<jats:sup>+<\/jats:sup> and ~50% CD8<jats:sup>+<\/jats:sup> human T cells. The number of skin\u2010resident macrophages or dendritic cells was unaltered compared to healthy skin prior to transplantation, and PBMC administration did not alter their numbers. Together, we show that parasitic infection provides a strong inflammatory signal that leads to recruitment of T cells into skin transplants. The presence of antigen\u2010presenting cells in the transplants\u2014as an important prerequisite for proper APC\u2010T\u2010cell interaction\u2014recreates a fully human skin microenvironment that allows for stroma\/immune cell interactions upon infection. This model may be of high interest to researchers interested in translating skin research questions into the human system in\u00a0vivo.<\/jats:p>","DOI":"10.1111\/exd.70131","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T06:25:37Z","timestamp":1751351137000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Humanised Mice in Cutaneous Leishmaniasis\u2014T\u2010Cell Recruitment Into Human Skin Transplants After <scp><i>Leishmania major<\/i><\/scp> Infection"],"prefix":"10.1111","volume":"34","author":[{"given":"Ling","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine University of Cologne  Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henning","family":"Klapproth","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine University of Cologne  Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Stepkes","sequence":"additional","affiliation":[{"name":"Department of Dermatology Johannes Gutenberg University  Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Wegner","sequence":"additional","affiliation":[{"name":"Department of Dermatology Johannes Gutenberg University  Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9802-9642","authenticated-orcid":false,"given":"Esther","family":"von Stebut","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine University of Cologne  Cologne Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri933"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13999"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030159"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001491"},{"issue":"3","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/ddg.12595","article-title":"Leishmaniasis","volume":"13","author":"Stebut E.","year":"2015","journal-title":"Journal Der Deutschen Dermatologischen Gesellschaft"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031482"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3661-8_9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)00002-3"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T17:14:53Z","timestamp":1758734093000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.70131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":8,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/exd.70131"],"URL":"https:\/\/doi.org\/10.1111\/exd.70131","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"type":"print","value":"0906-6705"},{"type":"electronic","value":"1600-0625"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70131"}}