{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:29:01Z","timestamp":1792985341617,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100022756","name":"Centre for Human Drug Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100022756","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017559","name":"Recordati Rare Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017559","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["NWA.1389.20.182"],"award-info":[{"award-number":["NWA.1389.20.182"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The composite index lesion severity (CAILS) score is used to monitor disease and therapeutic response in mycosis fungoides (MF), but is limited by interobserver variability and low sensitivity. Emerging imaging techniques, such as multispectral imaging (MSI), colourimetry and laser speckle contrast imaging (LSCI), offer objective alternatives for quantifying CAILS parameters. The aim of this study was to evaluate non\u2010invasive imaging modalities for objective and reliable quantification of disease extent in MF. Sixty\u2010six participants were enrolled in two prospective studies: a cross\u2010sectional discovery cohort to assess baseline characteristics of 35 MF patients (IA\u2013IVB) and 10 healthy controls using CAILS and MSI, and a longitudinal confirmation cohort including 21 early\u2010stage MF patients (IA\u2013IIA) treated with chlormethine gel 0.016% for 16\u2009weeks, in whom lesional and non\u2010lesional skin were assessed using CAILS, MSI, colourimetry and LSCI at multiple time points. Candidate biomarkers were required to meet five clinical validation criteria: disease discrimination, repeatability, treatment responsiveness, correlation with CAILS and patient acceptability. In the discovery cohort, MSI detected significant differences in erythema, pigmentation, elevation and desquamation between healthy, non\u2010lesional and lesional skin. In the confirmation cohort, four candidate biomarkers met all validation criteria: MSI CIELAB a*, MSI average haemoglobin, and colourimetry CIELAB a* (DSMIII) for quantifying erythema, and MSI individual typology angle (ITA) for pigmentation. These biomarkers reliably discriminated lesional from non\u2010lesional skin (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.001), showed strong test\u2013retest reliability (CV &lt;\u200910%, ICC &gt;\u20090.84), detected treatment effects, showed moderate concordance with CAILS, and were associated with low patient burden (mean 3.4\/100). These findings show that MSI\u2010 and colourimetry\u2010derived biomarkers can objectively monitor disease extent in MF and complement existing clinical assessments.\n                  <\/jats:p>","DOI":"10.1111\/exd.70236","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T10:57:25Z","timestamp":1773226645000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Clinical Validation of Imaging Biomarkers in Mycosis Fungoides"],"prefix":"10.1111","volume":"35","author":[{"given":"Selinde S.","family":"Wind","sequence":"first","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1332-7061","authenticated-orcid":false,"given":"Elise S. M.","family":"Beljaards","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rianne","family":"Rijneveld","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Bruijnincx","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Leiden Academic Centre for Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessa Niemeyer\u2010van","family":"der Kolk","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9207-1807","authenticated-orcid":false,"given":"Manon A. A.","family":"Jansen","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalcin","family":"Yavuz","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marieke","family":"de Kam","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacobus","family":"Burggraaf","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"},{"name":"Leiden Academic Centre for Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Klarenbeek","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacobus","family":"Bosch","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen D.","family":"Quint","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten H.","family":"Vermeer","sequence":"additional","affiliation":[{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5867-9090","authenticated-orcid":false,"given":"Robert","family":"Rissmann","sequence":"additional","affiliation":[{"name":"Centre for Human Drug Research  Leiden the Netherlands"},{"name":"Department of Dermatology Leiden University Medical Center  Leiden the Netherlands"},{"name":"Leiden Academic Centre for Drug Research  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Next\u2010Generation ImmunoDermatology Consortium (NGID)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3502"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.12788\/j.sder.2018.002"},{"issue":"4","key":"e_1_2_12_4_1","first-page":"504","article-title":"Mycosis Fungoides: Disease Evolution and Prognosis of 309 Dutch Patients","volume":"136","author":"Doorn R.","year":"2000","journal-title":"Archives of Dermatology"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13555\u2010024\u201001305\u2010x"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.139.7.857"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-022-00732-w"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000472711"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/69804"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-03-055749"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-100-2-187"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70491-3"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-109-5-372"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2012.04499.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.17219"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.18078"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.5114\/ada.2017.72457"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2007.02.026"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jum.15297"},{"issue":"9","key":"e_1_2_12_20_1","article-title":"Morphologic Features of Cutaneous T\u2010Cell Lymphomas Using Dermoscopy and High\u2010Frequency Ultrasound","volume":"9","author":"Wohlmuth\u2010Wieser I.","year":"2020","journal-title":"Journal of Clinical Medicine"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257-023-00824-1"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.12049"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.19633"},{"key":"e_1_2_12_24_1","unstructured":"M. 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