{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T15:15:47Z","timestamp":1783437347758,"version":"3.54.6"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The hydroxycarboxylic acid receptor HCA2 is expressed in keratinocytes and immune cells. In mice, the anti\u2010inflammatory potential of HCA2 receptor signalling in the skin was first described in experimental models of psoriasis\u2010like inflammation and bullous pemphigoid\u2010like epidermolysis bullosa acquisita. We examined contact allergic immune responses to the obligate contact sensitiser DNFB in\n                    <jats:italic>Hcar2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    and wild\u2010type C57BL\/6 mice. Basal mRNA levels of pro\u2010inflammatory mediators like\n                    <jats:italic>IFN\u03b3<\/jats:italic>\n                    were already increased in the ear tissue of na\u00efve\n                    <jats:italic>Hcar2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    animals. After sensitisation and challenge with DNFB, contact allergic ear swelling and infiltration of neutrophils and CD3+ T cells were increased in\n                    <jats:italic>Hcar2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice. To investigate the impact of HCA2 receptors on T cells, we performed in\u00a0vitro co\u2010stimulation assays with allergen\u2010loaded dendritic cells and antigen\u2010specific T cells, showing increased proliferation and IFN\u03b3 production of\n                    <jats:italic>Hcar2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    T lymphocytes. Adoptive transfer of sensitised lymphocytes and experiments with bone marrow chimeric mice indicated that HCAR2 exerts its anti\u2010inflammatory effect in part through radio\u2010resistant, skin\u2010resident cells in the challenge phase. As a potential mechanism, we found that\n                    <jats:italic>Hcar2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    keratinocytes produced higher levels of the neutrophil\u2010attracting chemokine CCL8. In summary, we show that HCA2 receptors are functionally expressed in lymphocytes and keratinocytes and participate in the attenuation of contact allergic immune responses. Our data indicate that the dominant anti\u2010inflammatory effect of HCA2 signalling during the elicitation phase resides in radio\u2010resistant, skin\u2010resident cells, whereas effects on lymphocyte activation are likely to be modulatory. The precise contribution of distinct skin\u2010resident cell populations and the role of endogenous ligands driving HCA2 signalling in this context remain unresolved.\n                  <\/jats:p>","DOI":"10.1111\/exd.70254","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T05:11:37Z","timestamp":1777353097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>HCA2<\/scp>\n                    Receptors in Lymphocytes and Keratinocytes Affect Murine Contact Allergic Inflammation"],"prefix":"10.1111","volume":"35","author":[{"given":"Sina","family":"Polkownik","sequence":"first","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Meens","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagen","family":"Lange","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7135-9731","authenticated-orcid":false,"given":"Johannes","family":"Weil","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"Kruse","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Dermatology, Department of Dermatology University Hospital Magdeburg  Magdeburg 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