{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T04:57:51Z","timestamp":1780981071721,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:00:00Z","timestamp":1778198400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32AI083216\u201015"],"award-info":[{"award-number":["T32AI083216\u201015"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K23AI171085"],"award-info":[{"award-number":["K23AI171085"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Bullous pemphigoid (BP) is an autoimmune blistering disease whereby the cutaneous antigens BP180 and BP230 are targeted by autoantibodies. Skin lesions in BP are characterized by an abundance of eosinophils. We recently demonstrated that the treatment of keratinocytes with antibodies from patients with BP induces a robust inflammatory response with release of numerous cytokines, chemokines, complement factors, and proteases. We thus questioned whether this keratinocyte inflammatory response was capable of directly inducing an inflammatory response in eosinophils. We therefore treated human eosinophils with conditioned media from keratinocytes treated with IgG from patients with BP (BP\u2010IgG), or healthy controls (control\u2010IgG) with or without supplemental IL\u20105. Flow cytometry revealed upregulation of CD107a\/CD107b, markers of degranulation, on eosinophils treated with supernatants from BP\u2010IgG relative to control\u2010IgG treated keratinocytes. A decrease in CCR3 and CD101 was also identified. Functional activity of eosinophils was confirmed by performing a multiplex immunoassay on eosinophil supernatants. This revealed significant upregulation IL\u20106, IL\u20108, LIF, TGF\u03b1, MCP\u20104, MMP\u20109, and MMP\u201010. Supplemental IL\u20105 did not appear to significantly influence these responses. Our data demonstrate that the inflammatory activation of keratinocytes by BP\u2010IgG affects eosinophils, driving phenotypic changes consistent with degranulation, as well as a pro\u2010inflammatory and proteolytic response.<\/jats:p>","DOI":"10.1111\/exd.70265","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T11:45:03Z","timestamp":1778240703000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Secretome of Bullous Pemphigoid\n                    <scp>IgG<\/scp>\n                    \u2010Treated Keratinocytes Induces a Pro\u2010Inflammatory Eosinophil Response"],"prefix":"10.1111","volume":"35","author":[{"given":"Adrian P.","family":"Mansini","sequence":"first","affiliation":[{"name":"Department of Dermatology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Dermatology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishan D.","family":"Chhiba","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1701-6304","authenticated-orcid":false,"given":"Yulu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7537-1787","authenticated-orcid":false,"given":"Haley","family":"Gainer","sequence":"additional","affiliation":[{"name":"Department of Dermatology Rush University Medical Center  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Allen","family":"McAlexander","sequence":"additional","affiliation":[{"name":"Translational Science and Experimental Medicine, Early Respiratory &amp; Immunology AstraZeneca  Gaithersburg Maryland USA"},{"name":"Translational Science Johnson and Johnson Innovative Medicine  Spring House Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7184-9069","authenticated-orcid":false,"given":"Christopher","family":"McCrae","sequence":"additional","affiliation":[{"name":"Translational Science and Experimental Medicine, Early Respiratory &amp; Immunology AstraZeneca  Gaithersburg Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Nazaroff","sequence":"additional","affiliation":[{"name":"Translational Science and Experimental Medicine, Early Respiratory &amp; Immunology AstraZeneca  Gaithersburg Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei Li","family":"Kuang","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology Northwestern University Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2906-2454","authenticated-orcid":false,"given":"Kyle T.","family":"Amber","sequence":"additional","affiliation":[{"name":"Department of Dermatology Rush University Medical Center  Chicago Illinois USA"},{"name":"Department of Ophthalmology Rush University Medical Center  Chicago Illinois 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