{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T15:17:15Z","timestamp":1789831035615,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 22K08438"],"award-info":[{"award-number":["JP 22K08438"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The dermal papilla (DP) is essential to hair follicle development and regeneration. Isolation of human DPs still largely depends on manual microdissection and human DP cells (DPCs) lose their intrinsic properties in\u00a0vitro. Establishing a culture condition that maintains the biological properties of DPCs allows for identification of cell surface markers that enable cell sorting of living human DPCs. A new DPC culture condition was developed using the combination of a WNT activator, CHIR99021, and recombinant FGF9 (CH\u2009+\u2009F9). Global gene expression profiling and bioinformatic analyses of DPCs grown under this condition were conducted to identify DPC surface markers enabling live DPC isolation. Compared to a conventional culture condition, CH\u2009+\u2009F9 increased the expression of the representative DPC biomarkers\n                    <jats:italic>WNT5A<\/jats:italic>\n                    ,\n                    <jats:italic>LEF1 and BMP4<\/jats:italic>\n                    by 3.4\u2010, 3.8\u2010 and 60.5\u2010fold (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) and better maintained their expression levels after long\u2010term serial passaging. Aggregated CH\u2009+\u2009F9\u2010treated DPCs unevenly upregulated DP biomarkers further, suggesting that highly potent DPCs can be enriched using a marker for such cells. Bioinformatics analysis of CH\u2009+\u2009F9\u2010treated and control DPCs identified cell marker candidates, including roundabout guidance receptor 2 (ROBO2). Importantly, ROBO2\n                    <jats:sup>+<\/jats:sup>\n                    DPCs expressing the representative DP biomarkers\n                    <jats:italic>WNT5A<\/jats:italic>\n                    ,\n                    <jats:italic>VCAN<\/jats:italic>\n                    ,\n                    <jats:italic>NOG<\/jats:italic>\n                    were successfully sorted from mixed cell suspensions of keratinocytes, fibroblasts and DPCs mimicking enzymatically dissociated human skin. These findings suggest that the new culture condition and cell surface marker for human DPCs established in this study provide useful tools for drug discovery and regenerative medicine to address hair loss diseases.\n                  <\/jats:p>","DOI":"10.1111\/exd.70266","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:53:09Z","timestamp":1781265189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of\n                    <scp>ROBO2<\/scp>\n                    as a Useful Cell Surface Marker for Live Human Dermal Papilla Cell Isolation Utilizing a Novel Culture Condition With\n                    <scp>WNT<\/scp>\n                    and\n                    <scp>FGF<\/scp>\n                    Signalling Activation"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2634-8650","authenticated-orcid":false,"given":"Reina","family":"Hayakawa","sequence":"first","affiliation":[{"name":"Department of Dermatology Kyorin University School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5360-4313","authenticated-orcid":false,"given":"Ryo","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility Kyorin University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2211-4127","authenticated-orcid":false,"given":"Masahiro","family":"Fukuyama","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kyorin University School of Medicine  Tokyo 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