{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:04:55Z","timestamp":1785434695507,"version":"3.56.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8256120558"],"award-info":[{"award-number":["8256120558"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260615"],"award-info":[{"award-number":["82260615"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360458"],"award-info":[{"award-number":["82360458"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ultraviolet B (UVB) irradiation initiates cutaneous vitamin D\u2010related photochemistry from 7\u2010dehydrocholesterol (7\u2010DHC), which is also the immediate precursor of cholesterol via 7\u2010dehydrocholesterol reductase (DHCR7). Thus, DHCR7 occupies a branch\u2010point position linking cholesterol biosynthesis and UVB\u2010associated vitamin D\u2010related metabolism. How keratinocytes regulate this metabolic relationship under UVB remains unclear. We examined whether OPN1SW is associated with DHCR7 protein abundance, conditioned medium 25\u2010hydroxyvitamin D3 [25(OH)D3] (a vitamin D\u2010related readout) and sterol\u2010pool responses in UVB\u2010exposed keratinocytes. A UVB dose that preserved &gt;\u200980% cell viability, 10\u2009mJ\/cm\n                    <jats:sup>2<\/jats:sup>\n                    , increased OPN1SW protein abundance and reduced DHCR7 protein abundance in human epidermal keratinocytes and HaCaT cells. These changes were accompanied by increased conditioned medium 25(OH)D3 and a reduced cellular sterol\u2010pool readout. OPN1SW overexpression increased DHCR7 protein abundance under basal conditions. Under UVB exposure, OPN1SW overexpression attenuated UVB\u2010associated DHCR7 reduction, attenuated the UVB\u2010associated increase in conditioned medium 25(OH)D3 and partially preserved the sterol\u2010pool readout. Conversely, OPN1SW knockdown exacerbated DHCR7 reduction under UVB and was accompanied by higher conditioned medium 25(OH)D3 and a lower sterol\u2010pool readout. DHCR7 knockdown produced concordant shifts in these readouts, supporting a contributory role for DHCR7. Together, these findings support the presence of a UVB\u2010responsive OPN1SW\u2010DHCR7 module that may contribute to keratinocyte adaptation to UVB exposure.\n                  <\/jats:p>","DOI":"10.1111\/exd.70285","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T10:43:07Z","timestamp":1780310587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>OPN<\/scp>\n                    1\n                    <scp>SW<\/scp>\n                    Modulates\n                    <scp>UVB<\/scp>\n                    \u2010Associated\n                    <scp>DHCR<\/scp>\n                    7 Reduction and Associated 25(\n                    <scp>OH<\/scp>\n                    )\n                    <scp>D<\/scp>\n                    3 and Sterol\u2010Pool Responses in Keratinocytes"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6503-4494","authenticated-orcid":false,"given":"Yating","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology, College of Clinical Medicine Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8251-4624","authenticated-orcid":false,"given":"Wen","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, College of Clinical Medicine Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Dermatology Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3984-9261","authenticated-orcid":false,"given":"Guangsu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, College of Clinical Medicine Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1796-0182","authenticated-orcid":false,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, College of Clinical Medicine Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Dermatology Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4984-8443","authenticated-orcid":false,"given":"Yushen","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, College of Clinical Medicine Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5002-4276","authenticated-orcid":false,"given":"Hongguang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, College of Clinical Medicine Guizhou Medical University  Guiyang Guizhou China"},{"name":"Department of Dermatology Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2007.03.004"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/derm.24494"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2014.12.026"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-019-0312-5"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2024.04.022"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2026.117711"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202503003R"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2019.105449"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2016.09.003"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40200-020-00561-w"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.11.007"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43892-x"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2016.05.003"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2008.10"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.05.017"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5480.739"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2019.100668"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.787730"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12354"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.08.063"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-04621-8"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2017.07.833"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2308374121"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.699546"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.09.008"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj439"}],"container-title":["Experimental 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