{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:07:28Z","timestamp":1785434848772,"version":"3.56.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The circadian clock regulates multiple physiological processes in the skin, including local hormone synthesis and pigmentation. However, how circadian regulation interacts with epidermal endocrine signalling in melanocytes remains unclear. In this study, we investigated whether core circadian regulators influence melanogenesis in human melanocytes and explored their potential link to epidermal endocrine factors and pigmentary disease. Primary normal human epidermal melanocytes were analysed following siRNA\u2010mediated knockdown of the core clock genes basic helix\u2013loop\u2013helix ARNT\u2010like protein 1 (BMAL1) and CLOCK circadian regulator (CLOCK). Silencing either gene significantly reduced melanin synthesis and altered the expression of hormone\u2010related factors. Notably, expression of the vitamin D\u2010metabolizing enzyme CYP27A1 was markedly decreased after BMAL1 or CLOCK knockdown, changes that were associated with reduced melanogenic activity. Consistently, immunohistochemical analysis revealed diminished CYP27A1 expression in melanocytes within vitiligo lesions. These findings demonstrate that disruption of circadian clock regulators impairs melanogenesis and is accompanied by alterations in vitamin D\u2010related components. Our results suggest that circadian regulation may modulate pigmentation partly through effects on vitamin D metabolism, providing new insight into the mechanisms underlying pigmentary disorders.<\/jats:p>","DOI":"10.1111\/exd.70296","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:37:43Z","timestamp":1781156263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Circadian Clock Regulates Epidermal Endocrine System in Homeostatic Skin Pigmentation"],"prefix":"10.1111","volume":"35","author":[{"given":"Anya","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pigmentation Research and Therapeutics, Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"},{"name":"College of Life Sciences Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingli","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pigmentation Research and Therapeutics, Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Pigmentation Research and Therapeutics, Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pigmentation Research and Therapeutics, Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"},{"name":"Cosmetic Products Research, Research and Development Kao Corporation  Odawara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6283-0251","authenticated-orcid":false,"given":"Daisuke","family":"Tsuruta","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Katayama","sequence":"additional","affiliation":[{"name":"Department of Pigmentation Research and Therapeutics, Graduate School of Medicine Osaka Metropolitan University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"issue":"2","key":"e_1_2_12_2_1","first-page":"85","article-title":"Overview of Circadian Rhythms","volume":"25","author":"Vitaterna M. 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