{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:07:26Z","timestamp":1785434846924,"version":"3.56.0"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["L234068"],"award-info":[{"award-number":["L234068"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273555"],"award-info":[{"award-number":["82273555"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Necroptosis, a form of programmed cell death, promotes inflammation in immune\u2010mediated diseases, but its role in alopecia areata (AA) remains unclear. In this study, we investigated necroptosis\u2010related signatures in AA using integrated transcriptomic and histological analyses. Four bulk RNA\u2010seq datasets from the GEO database, comprising 191 scalp samples from AA patients and healthy controls, were analysed by ssGSEA and GSEA to assess necroptosis\u2010related signatures. To address the cellular heterogeneity inherent in bulk RNA data, we further performed single\u2010cell RNA\u2010sequencing (scRNA\u2010seq) to investigate cell\u2010specific mechanisms, and immunofluorescence staining of scalp biopsies was conducted to validate the expression and cellular localisation of key necroptosis\u2010related markers. Necroptosis\u2010related scores were significantly higher in AA than in controls, correlated with disease severity and decreased after JAK\/TYK2 inhibitor therapy. At single\u2010cell resolution, a macrophage subset (IL4I1\n                    <jats:sup>+<\/jats:sup>\n                    macrophages_1) showed the strongest necroptosis\u2010related signal and was associated with enhanced macrophage\u2013fibroblast communication involving TGF\u03b2, ITGB2 and GAS6 signalling, suggesting perifollicular microenvironmental remodelling. Immunofluorescence further supported increased necroptosis\u2010associated signalling and macrophage enrichment in AA lesions. Together, these findings suggest that macrophage\u2010associated necroptosis\u2010related programmes may represent a disease\u2010associated inflammatory component in AA and support further mechanistic investigation of necroptosis\u2010associated pathways in this disease.\n                  <\/jats:p>","DOI":"10.1111\/exd.70305","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T09:41:24Z","timestamp":1782121284000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Analysis of Macrophage\u2010Associated Necroptosis in Alopecia Areata"],"prefix":"10.1111","volume":"35","author":[{"given":"Lin","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Dermatology Beijing Friendship Hospital, Capital Medical University  Beijing 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