{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:50:25Z","timestamp":1787323825919,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Human\u2010derived exosomes show promise for hair loss treatment but may pose safety concerns, including inflammatory responses and pathogen transmission. Here, we investigated whether plant\u2010derived exosomes, whose isolation can be standardized, and which show lower compositional complexity and no immunogenicity, represent a valid alternative. We isolated and characterized exosome\u2010like nanovesicles from the Ashwagandha plant (Ash\u2010ELNs), which is traditionally used to relieve stress, and pre\u2010clinically explored their hair growth\u2010promoting potential. Multi\u2010omic approaches demonstrated low variations in the content of seven different Ash\u2010ELN preparations, and cellular vesicle uptake was confirmed in human dermal fibroblasts (HDFs). In a scratch assay of human dermal papilla fibroblasts (HDPCs), Ash\u2010ELNs significantly increased the production\/secretion of the hair growth promoters VEGF\u2010A, PLGF1, FGF2 and LIF. Application of Ash\u2010ELNs to healthy human microdissected hair follicles significantly prolonged anagen and increased hair shaft production, while hair matrix keratinocyte proliferation and \u2010apoptosis remained unaltered. Ash\u2010ELNs significantly upregulated VEGF\u2010A protein expression in the bulb mesenchyme, corroborating our in\u00a0vitro results. Finally, Ash\u2010ELNs promoted tubulogenesis in an HDF\u2013human umbilical vein endothelial cell (HUVEC) assay, suggesting VEGF\u2010A\u2010induced angiogenesis as a possible mechanism underlying the anagen promoting effect of Ash\u2010ELN. Thus, Ash\u2010ELN treatment offers a novel adjuvant strategy or non\u2010drug agent to reduce hair shedding.<\/jats:p>","DOI":"10.1111\/exd.70315","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T08:08:09Z","timestamp":1784880489000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Treatment With Ashwagandha\u2010Derived Exosome\u2010Like Nanovesicles Up\u2010Regulates\n                    <scp>VEGF<\/scp>\n                    \u2010A Production and Promotes Human Hair Growth Ex\u00a0Vivo"],"prefix":"10.1111","volume":"35","author":[{"given":"Aliz\u00e9e","family":"Le Riche","sequence":"first","affiliation":[{"name":"QIMA Life Sciences QIMA Monasterium GmbH  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janina","family":"Nienhaus","sequence":"additional","affiliation":[{"name":"QIMA Life Sciences QIMA Monasterium GmbH  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Silva e Sousa","sequence":"additional","affiliation":[{"name":"QIMA Life Sciences QIMA Monasterium GmbH  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanieh","family":"Erdmann","sequence":"additional","affiliation":[{"name":"Hautarztpraxis Dr. Pajouh  Bargteheide Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Piccini","sequence":"additional","affiliation":[{"name":"QIMA Life Sciences QIMA Monasterium GmbH  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4117-3499","authenticated-orcid":false,"given":"Luisa Z.","family":"Collins","sequence":"additional","affiliation":[{"name":"Unilever Research &amp; Development  Wirral UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Barfoot","sequence":"additional","affiliation":[{"name":"Unilever Research &amp; Development  Wirral UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro L.","family":"Oseliero\u2010Filho","sequence":"additional","affiliation":[{"name":"Material Innovation Factory University of Liverpool  Merseyside UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepikka","family":"Senthilkumar","sequence":"additional","affiliation":[{"name":"Centre for Integrative Biology and Systems medicinE (IBSE) Indian Institute of Technology Madras (IITM)  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiyappan","family":"Lakshmanan","sequence":"additional","affiliation":[{"name":"Centre for Integrative Biology and Systems medicinE (IBSE) Indian Institute of Technology Madras (IITM)  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Bertolini","sequence":"additional","affiliation":[{"name":"QIMA Life Sciences QIMA Monasterium GmbH  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janin","family":"Edelkamp","sequence":"additional","affiliation":[{"name":"QIMA Life Sciences QIMA Monasterium GmbH  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Dell\u2018Acqua","sequence":"additional","affiliation":[{"name":"Nutraceutical Wellness Inc.  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