{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:50:21Z","timestamp":1787323821395,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515010768"],"award-info":[{"award-number":["2022A1515010768"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Resiniferatoxin (RTX) is a key tool for modelling pain\u2010induced white hair because it induces an acute pain\u2010related stress response. However, the specific dosage, injection method, and localization of white hair formation remain unclear. We aimed to investigate RTX application to establish a more stable mouse model of pain\u2010induced stress\u2010associated premature greying using a multidimensional approach. The effects of sex, age, RTX dose, depilation, and injection method on white hair location and area were investigated. Melanin granules and tyrosine\u2010related protein 2 (TRP2)\u2010positive cells in hair follicles were detected using histological and immunofluorescence analyses. RTX at 90\u2009\u03bcg\/kg induced the largest and most stable white hair area without inducing mortality. Male mice exhibited a higher proportion of white hair area compared to females. The proportion of white hair area was higher in 4\u2010week\u2010old male mice than in other age groups. Total and central activity distance ratios decreased in the open\u2010field tests. Weight gain slowed significantly. Haematoxylin and eosin and Fontana\u2013Masson staining revealed fewer melanin granules in the hair follicles and root areas of white hair. Immunofluorescence analysis showed a reduced TRP2\u2010positive cell count in white hair follicles. A model of pain\u2010induced hair depigmentation in 4\u2010week\u2010old male C57BL\/6J mice was established by intraperitoneal injection of RTX at 90\u2009\u03bcg\/kg. Wax hair removal on day 14 accelerated white hair formation, resulting in a stable white hair area with reduced melanin granules in the follicles.<\/jats:p>","DOI":"10.1111\/exd.70319","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:42:38Z","timestamp":1784292158000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Construction of a Modified Pain\u2010Induced Stress White Hair Model"],"prefix":"10.1111","volume":"35","author":[{"given":"Xunhong","family":"Xu","sequence":"first","affiliation":[{"name":"School of Public Health Southern Medical University  Guangzhou China"},{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenhua","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yarui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qimei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenhui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Public Health Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9391-2911","authenticated-orcid":false,"given":"Xueer","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8249-7174","authenticated-orcid":false,"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health Southern Medical University  Guangzhou China"},{"name":"Department of Histology and Embryology, School of Basic Medical Sciences Southern Medical University  Guangzhou China"},{"name":"NMPA Key Laboratory for Safety Evaluation of Cosmetics, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202403343R"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14264"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-1935-3"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.70126"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2024.149564"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0202955"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2021.11.040"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.148056"},{"key":"e_1_2_11_10_1","first-page":"1824","article-title":"Depilatory Creams Increase the Number of Hair Follicles, and Dermal Fibroblasts Expressing Interleukin\u20106, Tumor Necrosis Factor\u2010\u03b1, and Tumor","volume":"142","author":"Tsai P. F.","year":"2021","journal-title":"Korean Journal of Physiology & Pharmacology"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-40182-w"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106919"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.14014"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2022.101571"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2023.105412"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2023.04.015"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/md16060195"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705234"},{"key":"e_1_2_11_19_1","article-title":"Establishing a Mouse Model of a Pure Small Fiber Neuropathy With the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1","volume":"132","author":"Lee Y. C.","year":"2018","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-015-0875-z"},{"key":"e_1_2_11_21_1","volume-title":"Expression and Localization of FGF20 and Its Receptors FGFR2 and FGFR3 in the Formation and Development of Mouse Hair Follicles","author":"Shu N.","year":"2018"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40122-013-0015-x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.156"},{"key":"e_1_2_11_24_1","volume-title":"The Role of FGF7 Subfamily in the Formation and Development of Mouse Hair Follicles","author":"Jiaqi C.","year":"2017"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3319"},{"key":"e_1_2_11_26_1","first-page":"34","article-title":"Establishment of a Hair Cycle Animal Model in C57BL\/6J Mice","volume":"04","author":"Shuxia Y.","year":"1999","journal-title":"Chinese Journal of Dermatology"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.2009_2467"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70319","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.70319","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70319","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:14:11Z","timestamp":1787321651000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.70319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/exd.70319"],"URL":"https:\/\/doi.org\/10.1111\/exd.70319","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70319"}}