{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:50:56Z","timestamp":1787323856034,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:00:00Z","timestamp":1784246400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Type VII collagen (COL7), a major component of anchoring fibrils, is essential for dermal\u2010epidermal adhesion and pathogenic variants in\n                    <jats:italic>COL7A1<\/jats:italic>\n                    , encoding COL7, cause dystrophic epidermolysis bullosa (DEB). In normal skin, COL7 is localised just beneath the lamina densa. In contrast, previous immunoelectron microscopy (IEM) studies of DEB skin with residual COL7 expression demonstrated aberrant localisation of COL7 above the lamina densa and hemidesmosomes of epidermal basal keratinocytes, possibly reflecting impaired secretion of defective COL7. However, conventional microscopy techniques lack sufficient spatial resolution to clearly visualise individual protein distributions and resolve this finding. Here, we revisit the abnormal localisation of COL7 using two higher\u2010resolution approaches: structured illumination microscopy (SIM) and expansion microscopy (ExM). In normal human skin, the COL7 NC1 domain colocalised with type IV collagen (COL4), a surrogate marker of the lamina densa and was consistently detected beneath integrin \u03b16 (ITGA6), a hemidesmosomal marker. In DEB skin with residual COL7 expression, COL7 was detected above ITGA6 within basal keratinocytes, recapitulating previous IEM observations. SIM quantitatively confirmed significant BMZ disorganisation in DEB, whereas ExM showed a consistent but non\u2010significant trend, likely due to variability in expansion factors. These findings demonstrate that high\u2010resolution fluorescence imaging can reproduce classic IEM observations of COL7 mislocalisation, suggesting its potential as a more accessible complementary approach for morphologic assessment of DEB.\n                  <\/jats:p>","DOI":"10.1111\/exd.70330","type":"journal-article","created":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T02:39:43Z","timestamp":1784342383000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Revisiting Type\n                    <scp>VII<\/scp>\n                    Collagen Mislocalisation in Dystrophic Epidermolysis Bullosa Using High\u2010Resolution Imaging"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0075-0851","authenticated-orcid":false,"given":"Mika","family":"Watanabe","sequence":"first","affiliation":[{"name":"Department of Dermatology Faculty of Medicine and Graduate School of Medicine, Hokkaido University  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3166-8572","authenticated-orcid":false,"given":"Kazuki","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine and Graduate School of Medicine, Hokkaido University  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-1498","authenticated-orcid":false,"given":"Satoru","family":"Shinkuma","sequence":"additional","affiliation":[{"name":"Department of Dermatology Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3489-5418","authenticated-orcid":false,"given":"Hideyuki","family":"Ujiie","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine and Graduate School of Medicine, Hokkaido University  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3865-6366","authenticated-orcid":false,"given":"Ken","family":"Natsuga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine and Graduate School of Medicine, Hokkaido University  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018218"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2024.07.014"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/ds.DS-D-25-00149"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2011.01.010"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14222"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2025.02.001"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.18921"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCID.S54681"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2021.11.004"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2006.045302"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13023-022-02605-1"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb08546.x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000051637"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.24331"},{"issue":"6","key":"e_1_2_11_16_1","first-page":"753","article-title":"Most Anchoring Fibrils in Human Skin Originate and Terminate in the Lamina Densa","volume":"76","author":"Shimizu H.","year":"1997","journal-title":"Laboratory 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