{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:06:40Z","timestamp":1788934000047,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012331","name":"LEO Fondet","doi-asserted-by":"publisher","award":["LF\u2010OC\u201021\u2010000826"],"award-info":[{"award-number":["LF\u2010OC\u201021\u2010000826"]}],"id":[{"id":"10.13039\/501100012331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Cutibacterium acnes<\/jats:italic>\n                    is a common skin bacterium that inhabits sebaceous follicles and comprises multiple phylotypes. Healthy skin is colonized by a\n                    <jats:italic>C. acnes<\/jats:italic>\n                    population dominated by phylotypes IA, IB, and II. In acne, this diversity is reduced, with a predominance of phylotype IA. Quantifying\n                    <jats:italic>C. acnes<\/jats:italic>\n                    phylotypes has been challenging due to the lack of specific methods. Here, we developed a droplet digital PCR (ddPCR) assay that separately quantifies phylotype IA and phylotypes IB\/II, based on a difference in the hyaluronidase gene. The method was applied to skin swabs from 14 healthy individuals and 14 acne patients. Healthy skin contained nearly equal amounts of IA and IB\/II, whereas acne samples showed a 111\u2010fold predominance of IA over IB\/II, due to a marked depletion of IB\/II, instead of an increase of IA. After 4\u20136\u2009months of isotretinoin treatment,\n                    <jats:italic>C. acnes<\/jats:italic>\n                    levels decreased drastically, with phylotype IA reduced by 50\u2010fold. Twelve to 15\u2009months after treatment, both phylotypes rebounded, although IB\/II recovered only partially. Taken together, this ddPCR method can quantitatively distinguish the acne\u2010dominant phylotype IA from healthy skin\u2010associated phylotypes IB\/II, and is thus a useful tool for skin microbiome studies and specifically for assessing\n                    <jats:italic>C. acnes<\/jats:italic>\n                    dysbiosis in acne.\n                  <\/jats:p>","DOI":"10.1111\/exd.70347","type":"journal-article","created":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T13:24:31Z","timestamp":1786800271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Quantification of\n                    <i>Cutibacterium acnes<\/i>\n                    Phylotypes\n                    <scp>IA<\/scp>\n                    and\n                    <scp>IB<\/scp>\n                    \/\n                    <scp>II<\/scp>\n                    on Healthy and Acne\u2010Prone Human Skin"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1918-6974","authenticated-orcid":false,"given":"Cecilie","family":"Feidenhansl","sequence":"first","affiliation":[{"name":"Department of Biomedicine, Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9921-2075","authenticated-orcid":false,"given":"Erinda","family":"Rruci","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Faculty of Health Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7214-6306","authenticated-orcid":false,"given":"Nastassia","family":"Kn\u00f6dlseder","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Faculty of Health Aarhus University  Aarhus Denmark"},{"name":"Department of Medicine and Life Sciences Universitat Pompeu Fabra  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2434-8204","authenticated-orcid":false,"given":"Hans B.","family":"Lomholt","sequence":"additional","affiliation":[{"name":"Clinical Institute Aalborg University  Aalborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7433-0190","authenticated-orcid":false,"given":"Holger","family":"Br\u00fcggemann","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Faculty of Health Aarhus University  Aarhus 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