{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:43:13Z","timestamp":1793032993858,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    There is growing evidence that a dysbiosis of the skin microbiome has an impact on carcinogenesis in cutaneous squamous cell carcinoma. However, the role of the microbiome in basal cell carcinoma (BCC) remains unclear due to limited, inconsistent, ex\u00a0vivo studies. Here, we analysed the skin microbiome of patients with BCC, including the tumour lesion as well as perilesional skin from the same subject. Skin swabs from BCC and perilesional skin were taken prior to tumour surgery. After DNA extraction, we used a metabarcoding approach based on 16S rRNA gene amplicon sequencing to analyse the cutaneous bacterial communities. In total, 63 patients with BCC were included for analysis. Most tumour lesions were located on sun\u2010exposed, sebaceous areas such as the nose, cheeks, and forehead. The genera\n                    <jats:italic>Corynebacterium, Cutibacterium<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    predominated both in BCC and perilesional skin. However, the microbiome of BCC was reduced in overall bacterial richness, with a significant overabundance of amplicon sequence variants (ASV) assigned to the genus\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    , resulting in a reduced complexity in microbial networks. The dominating ASV assigned to\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    was also detected in perilesional skin regions, indicating that the microbial dysbiosis in BCC affected regions is caused by natural members of the healthy skin microbiome. The BCC microbiome is characterised by microbial dysbiosis, reflected by a reduced overall bacterial richness, fewer microbe\u2010microbe interactions and a significant over\u2010representation of\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    species compared with perilesional skin. Further long\u2010read metagenomics and cultivation\u2010based studies are needed to identify\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    species driving dysbiosis.\n                  <\/jats:p>","DOI":"10.1111\/exd.70371","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T06:02:26Z","timestamp":1792216946000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Basal Cell Carcinoma May Be Associated With\n                    <i>Staphylococcus<\/i>\n                    Dysbiosis"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8607-9367","authenticated-orcid":false,"given":"Justin G.","family":"Schlager","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergy University Hospital, Ludwig Maximilian University (LMU) Munich  Munich Germany"},{"name":"Department of Dermatology University Clinic of Navarra  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8706-1557","authenticated-orcid":false,"given":"Daniel","family":"Stiefel","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy University Hospital, Ludwig Maximilian University (LMU) Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6627-2509","authenticated-orcid":false,"given":"Kathrin","family":"Patzer","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy University Hospital, Ludwig Maximilian University (LMU) Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1671-1125","authenticated-orcid":false,"given":"Michael","family":"Schloter","sequence":"additional","affiliation":[{"name":"Research Unit Comparative Microbiome Analysis Helmholtz Munich  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8872-836X","authenticated-orcid":false,"given":"B\u00e4rbel U.","family":"Foesel","sequence":"additional","affiliation":[{"name":"Research Unit Molecular Epidemiology, Institute of Epidemiology Helmholtz Munich, German Research Center for Environmental Health  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3296-1101","authenticated-orcid":false,"given":"Nathalie M.","family":"Zelzer","sequence":"additional","affiliation":[{"name":"Institute of Molecular Cardiovascular Research (IMCAR), Medical Faculty RWTH Aachen University  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars E.","family":"French","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy University Hospital, Ludwig Maximilian University (LMU) Munich  Munich Germany"},{"name":"Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine  Miami USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Gschwendtner","sequence":"additional","affiliation":[{"name":"Research Unit Comparative Microbiome Analysis Helmholtz Munich  Neuherberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy University Hospital, Ludwig Maximilian University (LMU) Munich  Munich Germany"},{"name":"Klinik F\u00fcr Dermatologie Und Allergologie M\u00fcnchen Klinik gGmbH  M\u00fcnchen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.10830.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2025.1276"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2019.2996"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0000000000000267"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2023.113254"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2023.0056"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2013.5870"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15030801"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2006.08.039"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djs298"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2024.1381492"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0905"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01432-18"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2022.03.017"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04401-22"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14092143"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa001999"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32610"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2019.102673"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.06.025"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.789042"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12030541"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.19887"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02224"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17041-7"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1009442"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbaa290"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004226"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1214\/009053606000000281"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002687"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_10_34_1","unstructured":"A.Kassambara 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Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70371","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.70371","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70371","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:30:23Z","timestamp":1793032223000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.70371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":58,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/exd.70371"],"URL":"https:\/\/doi.org\/10.1111\/exd.70371","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2025-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70371"}}