{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:45:28Z","timestamp":1792046728605,"version":"4.4.0"},"reference-count":96,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["2001514"],"award-info":[{"award-number":["2001514"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT2008928"],"award-info":[{"award-number":["GNT2008928"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Melanoma is a highly immunogenic malignancy, yet responses to systemic therapy remain variable. The tumour\u2010draining lymph node (\n                    <jats:styled-content style=\"fixed-case\">TDLN<\/jats:styled-content>\n                    ), including the sentinel lymph node (\n                    <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>\n                    ) during early regional spread, is not only a site of metastatic dissemination but also an important setting for T\u2010cell priming and immune regulation. Conventional classification of nodes as tumour\u2010positive or tumour\u2010negative provides essential staging information but reveals little about their immune state. Single\u2010cell immune transcriptomics, T\u2010cell receptor sequencing and single\u2010cell epigenomics now allow T\u2010cell states, clonal dynamics and regulatory programmes to be examined in greater detail. Spatial transcriptomics, multiplex imaging, spatial proteomics and emerging spatial metabolomics add the anatomical context needed to determine where these cells and molecular programmes occur within the intact tissue architecture. In this review, we examine how these approaches are being utilised to study T\u2010cell heterogeneity, spatial organisation and immune interactions within melanoma\n                    <jats:styled-content style=\"fixed-case\">TDLNs<\/jats:styled-content>\n                    , together with the emerging contribution of artificial intelligence (\n                    <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>\n                    )\u2010assisted pathology. We also critically evaluate how these technologies are applied, with attention to tissue requirements, spatial resolution, molecular coverage, computational demands, scalability to archival haematoxylin and eosin (H&amp;E) imaging cohorts and careful interpretation of predicted or inferred spatial features. By combining biological profiling with methodological appraisal, we highlight how multi\u2010omics immune signatures may refine nodal disease assessment and guide future biomarker development, while noting that clinical translation will require standardised analytical pipelines and disease\u2010specific validation across independent melanoma cohorts.\n                  <\/jats:p>","DOI":"10.1111\/exd.70376","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:08:12Z","timestamp":1792044492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatial and Single\u2010Cell Multi\u2010Omics Analyses of T\u2010Cell Subsets and Interactions in Melanoma Lymph Node Metastasis"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7098-5656","authenticated-orcid":false,"given":"Ras Azira","family":"Ramli","sequence":"first","affiliation":[{"name":"Faculty of Medicine Universiti Sultan Zainal Abidin  Kuala Terengganu Malaysia"},{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"},{"name":"QIMR Berghofer  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Tan","sequence":"additional","affiliation":[{"name":"Frazer Institute The University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9614-648X","authenticated-orcid":false,"given":"Yibi","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"},{"name":"QIMR Berghofer  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1385-529X","authenticated-orcid":false,"given":"Glen","family":"Boyle","sequence":"additional","affiliation":[{"name":"QIMR Berghofer  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7870-5703","authenticated-orcid":false,"given":"Quan","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"},{"name":"QIMR Berghofer  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"issue":"1","key":"e_1_2_13_2_1","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/s41392-023-01576-4","article-title":"Lymph Node Metastasis in Cancer Progression: Molecular Mechanisms, Clinical Significance and Therapeutic Interventions","volume":"8","author":"Ji H.","year":"2023","journal-title":"Signal Transduction and Targeted Therapy"},{"key":"e_1_2_13_3_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2023.115731","article-title":"Lymph Node Metastasis and Tumor\u2010Educated Immune Tolerance: Potential Therapeutic Targets Against Distant Metastasis","volume":"215","author":"Peng J.\u2010M.","year":"2023","journal-title":"Biochemical Pharmacology"},{"key":"e_1_2_13_4_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.semcdb.2015.01.008","article-title":"The Lymph Node Microenvironment and Its Role in the Progression of Metastatic Cancer","volume":"38","author":"Pereira E. R.","year":"2015","journal-title":"Seminars in Cell & Developmental Biology"},{"issue":"63","key":"e_1_2_13_5_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abg3551","article-title":"Tumor\u2010Draining Lymph Nodes: At the Crossroads of Metastasis and Immunity","volume":"6","author":"Bois H.","year":"2021","journal-title":"Science Immunology"},{"issue":"6","key":"e_1_2_13_6_1","first-page":"472","article-title":"Melanoma Staging: Evidence\u2010Based Changes in the American Joint Committee on Cancer Eighth Edition Cancer Staging Manual","volume":"67","author":"Gershenwald J. E.","year":"2017","journal-title":"CA: A Cancer Journal for Clinicians"},{"issue":"7","key":"e_1_2_13_7_1","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.183080","article-title":"Single\u2010Cell Transcriptomics of Melanoma Sentinel Lymph Nodes Identifies Immune Cell Signatures Associated With Metastasis","volume":"10","author":"Engelbrecht E.","year":"2025","journal-title":"JCI Insight"},{"issue":"8","key":"e_1_2_13_8_1","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.ccell.2022.06.009","article-title":"Immune Suppression in the Tumor\u2010Draining Lymph Node Corresponds With Distant Disease Recurrence in Patients With Melanoma","volume":"40","author":"Krimpen A.","year":"2022","journal-title":"Cancer Cell"},{"issue":"2","key":"e_1_2_13_9_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00262-020-02698-2","article-title":"Dissecting the Immune Landscape of Tumor Draining Lymph Nodes in Melanoma With High\u2010Plex Spatially Resolved Protein Detection","volume":"70","author":"Beasley G. M.","year":"2021","journal-title":"Cancer Immunology, Immunotherapy"},{"issue":"64","key":"e_1_2_13_10_1","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.abg7836","article-title":"A Reservoir of Stem\u2010Like CD8+ T Cells in the Tumor\u2010Draining Lymph Node Preserves the Ongoing Antitumor Immune Response","volume":"6","author":"Connolly K. A.","year":"2021","journal-title":"Science Immunology"},{"issue":"1","key":"e_1_2_13_11_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.trecan.2023.09.006","article-title":"The Tumor\u2010Draining Lymph Node as a Reservoir for Systemic Immune Surveillance","volume":"10","author":"Delclaux I.","year":"2024","journal-title":"Trends in Cancer"},{"issue":"1","key":"e_1_2_13_12_1","doi-asserted-by":"crossref","DOI":"10.3390\/lymphatics4010002","article-title":"CD8+ T Cell Dysfunction in Tumor\u2010Draining Lymph Nodes: A Hallmark of Tumor Immune Escape That May Arise Early During the Course of Cancer Progression","volume":"4","author":"Hargadon K. M.","year":"2026","journal-title":"Lymphatics"},{"issue":"10151","key":"e_1_2_13_13_1","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/S0140-6736(18)31559-9","article-title":"Melanoma","volume":"392","author":"Schadendorf D.","year":"2018","journal-title":"Lancet"},{"issue":"1","key":"e_1_2_13_14_1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13073-017-0424-2","article-title":"Analysis of 100,000 Human Cancer Genomes Reveals the Landscape of Tumor Mutational Burden","volume":"9","author":"Chalmers Z. R.","year":"2017","journal-title":"Genome Medicine"},{"issue":"3","key":"e_1_2_13_15_1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/exd.14741","article-title":"Melanoma Antigens Recognized by T Cells and Their Use for Immunotherapy","volume":"32","author":"Ohta S.","year":"2023","journal-title":"Experimental Dermatology"},{"issue":"25","key":"e_1_2_13_16_1","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1056\/NEJMc1713444","article-title":"Tumor Mutational Burden and Response Rate to PD\u20101 Inhibition","volume":"377","author":"Yarchoan M.","year":"2017","journal-title":"New England Journal of Medicine"},{"key":"e_1_2_13_17_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.1006665","article-title":"Genomic Landscape of the Immunogenicity Regulation in Skin Melanomas With Diverse Tumor Mutation Burden","volume":"13","author":"Georgoulias G.","year":"2022","journal-title":"Frontiers in Immunology"},{"issue":"1","key":"e_1_2_13_18_1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1056\/NEJMoa2109970","article-title":"Relatlimab and Nivolumab Versus Nivolumab in Untreated Advanced Melanoma","volume":"386","author":"Tawbi H. A.","year":"2022","journal-title":"New England Journal of Medicine"},{"issue":"7","key":"e_1_2_13_19_1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1056\/NEJMoa1310460","article-title":"Final Trial Report of Sentinel\u2010Node Biopsy Versus Nodal Observation in Melanoma","volume":"370","author":"Morton D. L.","year":"2014","journal-title":"New England Journal of Medicine"},{"issue":"19","key":"e_1_2_13_20_1","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1056\/NEJMoa1708539","article-title":"Adjuvant Dabrafenib Plus Trametinib in Stage III BRAF\u2010Mutated Melanoma","volume":"377","author":"Long G. V.","year":"2017","journal-title":"New England Journal of Medicine"},{"issue":"19","key":"e_1_2_13_21_1","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1056\/NEJMoa1802357","article-title":"Adjuvant Pembrolizumab Versus Placebo in Resected Stage III Melanoma","volume":"378","author":"Eggermont A. M.","year":"2018","journal-title":"New England Journal of Medicine"},{"issue":"10336","key":"e_1_2_13_22_1","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1016\/S0140-6736(22)00562-1","article-title":"Pembrolizumab Versus Placebo as Adjuvant Therapy in Completely Resected Stage IIB or IIC Melanoma (KEYNOTE\u2010716): A Randomised, Double\u2010Blind, Phase 3 Trial","volume":"399","author":"Luke J. J.","year":"2022","journal-title":"Lancet"},{"issue":"9","key":"e_1_2_13_23_1","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/nri1919","article-title":"Tumour\u2013Induced Immune Modulation of Sentinel Lymph Nodes","volume":"6","author":"Cochran A. J.","year":"2006","journal-title":"Nature Reviews Immunology"},{"issue":"9","key":"e_1_2_13_24_1","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.it.2023.07.002","article-title":"Melanoma\u2010Derived Mediators Can Foster the Premetastatic Niche: Crossroad to Lymphatic Metastasis","volume":"44","author":"Suman S.","year":"2023","journal-title":"Trends in Immunology"},{"issue":"2","key":"e_1_2_13_25_1","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12197","article-title":"Melanoma\u2010Derived Extracellular Vesicles Mediate Lymphatic Remodelling and Impair Tumour Immunity in Draining Lymph Nodes","volume":"11","author":"Leary N.","year":"2022","journal-title":"Journal of Extracellular Vesicles"},{"issue":"11","key":"e_1_2_13_26_1","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1038\/s41591-023-02583-2","article-title":"Adjuvant Nivolumab in Resected Stage IIB\/C Melanoma: Primary Results From the Randomized, Phase 3 CheckMate 76K Trial","volume":"29","author":"Kirkwood J. M.","year":"2023","journal-title":"Nature Medicine"},{"key":"e_1_2_13_27_1","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.trecan.2025.04.008","article-title":"Tumor\u2010Draining Lymph Nodes\u2013Friend or Foe During Immune Checkpoint Therapy?","volume":"11","author":"Renesse J.","year":"2025","journal-title":"Trends in Cancer"},{"issue":"5","key":"e_1_2_13_28_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.ccell.2020.09.001","article-title":"The PD\u20101\/PD\u2010L1\u2010Checkpoint Restrains T Cell Immunity in Tumor\u2010Draining Lymph Nodes","volume":"38","author":"Dammeijer F.","year":"2020","journal-title":"Cancer Cell"},{"key":"e_1_2_13_29_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1562797","article-title":"Immune Microenvironment of Tumor\u2010Draining Lymph Nodes: Insights for Immunotherapy","volume":"16","author":"Wei J.","year":"2025","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_13_30_1","doi-asserted-by":"crossref","unstructured":"I.Delclaux K. S.Ventre N. R.Besson et\u00a0al. \u201cLymphatic Egress Recycles Tumor\u2010Experienced Effector CD8 T Cells to Sustain Immune Surveillance \u201dbioRxiv(2026) https:\/\/doi.org\/10.64898\/2026.04.30.721705.","DOI":"10.64898\/2026.04.30.721705"},{"key":"e_1_2_13_31_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1568456","article-title":"Spatial Proteomics of the Tumor Microenvironment in Melanoma: Current Insights and Future Directions","volume":"16","author":"Bungaro C.","year":"2025","journal-title":"Frontiers in Immunology"},{"issue":"3","key":"e_1_2_13_32_1","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.cell.2020.04.017","article-title":"Dysfunctional CD8 T Cells Form a Proliferative, Dynamically Regulated Compartment Within Human Melanoma","volume":"181","author":"Li H.","year":"2020","journal-title":"Cell"},{"issue":"4","key":"e_1_2_13_33_1","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1016\/j.cell.2018.10.038","article-title":"Defining T Cell States Associated With Response to Checkpoint Immunotherapy in Melanoma","volume":"175","author":"Sade\u2010Feldman M.","year":"2018","journal-title":"Cell"},{"issue":"8","key":"e_1_2_13_34_1","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1038\/s41591-019-0522-3","article-title":"Clonal Replacement of Tumor\u2010Specific T Cells Following PD\u20101 Blockade","volume":"25","author":"Yost K. E.","year":"2019","journal-title":"Nature Medicine"},{"issue":"10","key":"e_1_2_13_35_1","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1016\/j.immuni.2024.08.014","article-title":"Tumor\u2010Reactive T Cell Clonotype Dynamics Underlying Clinical Response to TIL Therapy in Melanoma","volume":"57","author":"Chiffelle J.","year":"2024","journal-title":"Immunity"},{"issue":"3","key":"e_1_2_13_36_1","doi-asserted-by":"crossref","DOI":"10.1136\/jitc-2025-013499","article-title":"TCR Sequencing in Cancer Immunology and Immunotherapy: What, When, Where, Why, and How","volume":"14","author":"Nose Y.","year":"2026","journal-title":"Journal for Immunotherapy of Cancer"},{"issue":"6316","key":"e_1_2_13_37_1","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1126\/science.aaf2807","article-title":"Epigenetic Stability of Exhausted T Cells Limits Durability of Reinvigoration by PD\u20101 Blockade","volume":"354","author":"Pauken K. E.","year":"2016","journal-title":"Science"},{"issue":"1","key":"e_1_2_13_38_1","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.cell.2017.06.007","article-title":"De Novo Epigenetic Programs Inhibit PD\u20101 Blockade\u2010Mediated T Cell Rejuvenation","volume":"170","author":"Ghoneim H. E.","year":"2017","journal-title":"Cell"},{"issue":"8","key":"e_1_2_13_39_1","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1038\/s41587-019-0206-z","article-title":"Massively Parallel Single\u2010Cell Chromatin Landscapes of Human Immune Cell Development and Intratumoral T Cell Exhaustion","volume":"37","author":"Satpathy A. T.","year":"2019","journal-title":"Nature Biotechnology"},{"issue":"6","key":"e_1_2_13_40_1","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1158\/2159-8290.CD-21-1357","article-title":"The Spatial Landscape of Progression and Immunoediting in Primary Melanoma at Single\u2010Cell Resolution","volume":"12","author":"Nirmal A. J.","year":"2022","journal-title":"Cancer Discovery"},{"issue":"2","key":"e_1_2_13_41_1","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s41592-022-01728-4","article-title":"Screening Cell\u2013Cell Communication in Spatial Transcriptomics via Collective Optimal Transport","volume":"20","author":"Cang Z.","year":"2023","journal-title":"Nature Methods"},{"issue":"18","key":"e_1_2_13_42_1","doi-asserted-by":"crossref","first-page":"3275","DOI":"10.1158\/0008-5472.CAN-22-0363","article-title":"Mapping the Immune Landscape in Metastatic Melanoma Reveals Localized Cell\u2013Cell Interactions That Predict Immunotherapy Response","volume":"82","author":"Antoranz A.","year":"2022","journal-title":"Cancer Research"},{"issue":"6547","key":"e_1_2_13_43_1","doi-asserted-by":"crossref","DOI":"10.1126\/science.aba2609","article-title":"Analysis of Multispectral Imaging With the AstroPath Platform Informs Efficacy of PD\u20101 Blockade","volume":"372","author":"Berry S.","year":"2021","journal-title":"Science"},{"issue":"21","key":"e_1_2_13_44_1","doi-asserted-by":"crossref","first-page":"5250","DOI":"10.1158\/1078-0432.CCR-18-0309","article-title":"Quantitative Spatial Profiling of PD\u20101\/PD\u2010L1 Interaction and HLA\u2010DR\/IDO\u20101 Predicts Improved Outcomes of Anti\u2010PD\u20101 Therapies in Metastatic Melanoma","volume":"24","author":"Johnson D. B.","year":"2018","journal-title":"Clinical Cancer Research"},{"issue":"11","key":"e_1_2_13_45_1","doi-asserted-by":"crossref","DOI":"10.1002\/eji.70094","article-title":"Spatial Immunometabolism: Integrating Technologies to Decode Cellular Metabolism in Tissues","volume":"55","author":"Hartmann F. J.","year":"2025","journal-title":"European Journal of Immunology"},{"key":"e_1_2_13_46_1","doi-asserted-by":"crossref","unstructured":"M.Pang K.Su andM.Li \u201cLeveraging Information in Spatial Transcriptomics to Predict Super\u2010Resolution Gene Expression From Histology Images in Tumors \u201dbioRxiv(2021) https:\/\/doi.org\/10.1101\/2021.11.28.470212.","DOI":"10.1101\/2021.11.28.470212"},{"key":"e_1_2_13_47_1","doi-asserted-by":"crossref","first-page":"4225","DOI":"10.1016\/j.cell.2026.04.023","article-title":"AI\u2010Predicted Spatial Transcriptomics Unlocks Breast Cancer Biomarkers From Pathology","volume":"189","author":"Shulman E. D.","year":"2026","journal-title":"Cell"},{"issue":"7","key":"e_1_2_13_48_1","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1111\/imcb.70040","article-title":"Sketching T Cell Atlases in the Single\u2010Cell Era: Challenges and Recommendations","volume":"103","author":"Bojovi\u0107 I.","year":"2025","journal-title":"Immunology and Cell Biology"},{"issue":"7347","key":"e_1_2_13_49_1","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/nature10098","article-title":"Global Quantification of Mammalian Gene Expression Control","volume":"473","author":"Schwanh\u00e4usser B.","year":"2011","journal-title":"Nature"},{"issue":"1","key":"e_1_2_13_50_1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/s41597-024-03399-6","article-title":"Direct Comparison of Mass Cytometry and Single\u2010Cell RNA Sequencing of Human Peripheral Blood Mononuclear Cells","volume":"11","author":"Su E. Y.","year":"2024","journal-title":"Scientific Data"},{"issue":"5","key":"e_1_2_13_51_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.xgen.2025.100849","article-title":"Enhanced Interpretation of Immune Cell Phenotype and Function Through a Rhesus Macaque Single\u2010Cell Atlas","volume":"5","author":"Mahyari E.","year":"2025","journal-title":"Cell Genomics"},{"issue":"9","key":"e_1_2_13_52_1","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1038\/s41590-025-02241-4","article-title":"Multimodal Profiling Reveals Tissue\u2010Directed Signatures of Human Immune Cells Altered With Age","volume":"26","author":"Wells S. B.","year":"2025","journal-title":"Nature Immunology"},{"issue":"1","key":"e_1_2_13_53_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.crmeth.2024.100938","article-title":"Multimodal Hierarchical Classification of CITE\u2010Seq Data Delineates Immune Cell States Across Lineages and Tissues","volume":"5","author":"Caron D. P.","year":"2025","journal-title":"Cell Reports Methods"},{"issue":"8091","key":"e_1_2_13_54_1","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1038\/s41586-025-09539-1","article-title":"Proteotoxic Stress Response Drives T Cell Exhaustion and Immune Evasion","volume":"647","author":"Wang Y.","year":"2025","journal-title":"Nature"},{"issue":"4","key":"e_1_2_13_55_1","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1016\/j.ccell.2023.03.009","article-title":"Lineage Tracing Reveals Clonal Progenitors and Long\u2010Term Persistence of Tumor\u2010Specific T Cells During Immune Checkpoint Blockade","volume":"41","author":"Pai J. A.","year":"2023","journal-title":"Cancer Cell"},{"issue":"1","key":"e_1_2_13_56_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41592-020-01033-y","article-title":"Method of the Year: Spatially Resolved Transcriptomics","volume":"18","author":"Marx V.","year":"2021","journal-title":"Nature Methods"},{"issue":"5","key":"e_1_2_13_57_1","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/s41592-022-01409-2","article-title":"Museum of Spatial Transcriptomics","volume":"19","author":"Moses L.","year":"2022","journal-title":"Nature Methods"},{"issue":"1","key":"e_1_2_13_58_1","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1158\/2159-8290.CD-21-0316","article-title":"Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single\u2010Cell Level","volume":"12","author":"Wu Y.","year":"2022","journal-title":"Cancer Discovery"},{"issue":"6657","key":"e_1_2_13_59_1","doi-asserted-by":"crossref","DOI":"10.1126\/science.abq4964","article-title":"The Dawn of Spatial Omics","volume":"381","author":"Bressan D.","year":"2023","journal-title":"Science"},{"key":"e_1_2_13_60_1","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1038\/s41592-024-02565-3","article-title":"Method of the Year 2024: Spatial Proteomics","volume":"21","author":"Karimi E.","year":"2024","journal-title":"Nature Methods"},{"issue":"5","key":"e_1_2_13_61_1","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.ccell.2025.02.017","article-title":"Lymph Node Macrophages Drive Immune Tolerance and Resistance to Cancer Therapy by Induction of the Immune\u2010Regulatory Cytokine IL\u201033","volume":"43","author":"Lamorte S.","year":"2025","journal-title":"Cancer Cell"},{"issue":"3","key":"e_1_2_13_62_1","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.ccell.2022.01.002","article-title":"Tumor\u2010Associated High Endothelial Venules Mediate Lymphocyte Entry Into Tumors and Predict Response to PD\u20101 Plus CTLA\u20104 Combination Immunotherapy","volume":"40","author":"Asrir A.","year":"2022","journal-title":"Cancer Cell"},{"issue":"17","key":"e_1_2_13_63_1","first-page":"E4041","article-title":"Macrophages Impede CD8 T Cells From Reaching Tumor Cells and Limit the Efficacy of Anti\u2010PD\u20101 Treatment","volume":"115","author":"Peranzoni E.","year":"2018","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"4","key":"e_1_2_13_64_1","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/s41587-021-00830-w","article-title":"Robust Decomposition of Cell Type Mixtures in Spatial Transcriptomics","volume":"40","author":"Cable D. M.","year":"2022","journal-title":"Nature Biotechnology"},{"issue":"5","key":"e_1_2_13_65_1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/s41587-021-01139-4","article-title":"Cell2location Maps Fine\u2010Grained Cell Types in Spatial Transcriptomics","volume":"40","author":"Kleshchevnikov V.","year":"2022","journal-title":"Nature Biotechnology"},{"issue":"1","key":"e_1_2_13_66_1","doi-asserted-by":"crossref","DOI":"10.1186\/s13059-020-02048-6","article-title":"Systematic Assessment of Tissue Dissociation and Storage Biases in Single\u2010Cell and Single\u2010Nucleus RNA\u2010Seq Workflows","volume":"21","author":"Denisenko E.","year":"2020","journal-title":"Genome Biology"},{"key":"e_1_2_13_67_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13059-026-03937-y","article-title":"A Technical Comparison of Spatial Transcriptomics Platforms Across Six Cancer Types","volume":"27","author":"Cervilla S.","year":"2026","journal-title":"Genome Biology"},{"issue":"25","key":"e_1_2_13_68_1","doi-asserted-by":"crossref","first-page":"12410","DOI":"10.1073\/pnas.1905675116","article-title":"TOX and TOX2 Transcription Factors Cooperate With NR4A Transcription Factors to Impose CD8+ T Cell Exhaustion","volume":"116","author":"Seo H.","year":"2019","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"1","key":"e_1_2_13_69_1","doi-asserted-by":"crossref","first-page":"5440","DOI":"10.1038\/s41467-019-13043-2","article-title":"An Open Source Automated Tumor Infiltrating Lymphocyte Algorithm for Prognosis in Melanoma","volume":"10","author":"Acs B.","year":"2019","journal-title":"Nature Communications"},{"issue":"1","key":"e_1_2_13_70_1","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jaad.2023.09.026","article-title":"Automated Scoring of Tumor\u2010Infiltrating Lymphocytes Informs Risk of Death From Thin Melanoma: A Nested Case\u2010Case Study","volume":"90","author":"Tan S. X.","year":"2024","journal-title":"Journal of the American Academy of Dermatology"},{"issue":"1","key":"e_1_2_13_71_1","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1038\/s41467-025-67965-1","article-title":"sCellST Predicts Single\u2010Cell Gene Expression From H&E Images","volume":"17","author":"Chadoutaud L.","year":"2026","journal-title":"Nature Communications"},{"key":"e_1_2_13_72_1","doi-asserted-by":"crossref","first-page":"35796","DOI":"10.52202\/079017-1129","article-title":"STimage\u20101K4M: A Histopathology Image\u2010Gene Expression Dataset for Spatial Transcriptomics","volume":"37","author":"Chen J.","year":"2024","journal-title":"Advances in Neural Information Processing Systems"},{"issue":"7","key":"e_1_2_13_73_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114392","article-title":"Single\u2010Cell Spatial Multiomics Reveals Tumor Microenvironment Vulnerabilities in Cancer Resistance to Immunotherapy","volume":"43","author":"Quek C.","year":"2024","journal-title":"Cell Reports"},{"issue":"1","key":"e_1_2_13_74_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.cell.2023.11.037","article-title":"A TCF4\u2010Dependent Gene Regulatory Network Confers Resistance to Immunotherapy in Melanoma","volume":"187","author":"Pozniak J.","year":"2024","journal-title":"Cell"},{"issue":"5","key":"e_1_2_13_75_1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41591-018-0014-x","article-title":"Understanding the Tumor Immune Microenvironment (TIME) for Effective Therapy","volume":"24","author":"Binnewies M.","year":"2018","journal-title":"Nature Medicine"},{"issue":"6","key":"e_1_2_13_76_1","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1038\/s41591-019-0432-4","article-title":"Immune Induction Strategies in Metastatic Triple\u2010Negative Breast Cancer to Enhance the Sensitivity to PD\u20101 Blockade: The TONIC Trial","volume":"25","author":"Voorwerk L.","year":"2019","journal-title":"Nature Medicine"},{"issue":"1","key":"e_1_2_13_77_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/imr.12799","article-title":"Role of Lymph Node Stroma and Microenvironment in T Cell Tolerance","volume":"292","author":"Saxena V.","year":"2019","journal-title":"Immunological Reviews"},{"issue":"6","key":"e_1_2_13_78_1","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/j.immuni.2011.03.024","article-title":"Germinal Center B Cell and T Follicular Helper Cell Development Initiates in the Interfollicular Zone","volume":"34","author":"Kerfoot S. M.","year":"2011","journal-title":"Immunity"},{"issue":"5","key":"e_1_2_13_79_1","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.241828","article-title":"The Lymph Node at a Glance\u2013How Spatial Organization Optimizes the Immune Response","volume":"133","author":"Grant S. M.","year":"2020","journal-title":"Journal of Cell Science"},{"issue":"6","key":"e_1_2_13_80_1","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1038\/s43018-025-00963-w","article-title":"MCSP+ Metastasis Founder Cells Activate Immunosuppression Early in Human Melanoma Metastatic Colonization","volume":"6","author":"Guetter S.","year":"2025","journal-title":"Nature Cancer"},{"issue":"3","key":"e_1_2_13_81_1","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1038\/s41590-019-0312-6","article-title":"Subsets of Exhausted CD8+ T Cells Differentially Mediate Tumor Control and Respond to Checkpoint Blockade","volume":"20","author":"Miller B. C.","year":"2019","journal-title":"Nature Immunology"},{"issue":"6","key":"e_1_2_13_82_1","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.cell.2023.02.021","article-title":"Dynamic CD8+ T Cell Responses to Cancer Immunotherapy in Human Regional Lymph Nodes Are Disrupted in Metastatic Lymph Nodes","volume":"186","author":"Rahim M. K.","year":"2023","journal-title":"Cell"},{"key":"e_1_2_13_83_1","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2019.02876","article-title":"Role of Tumor\u2010Mediated Dendritic Cell Tolerization in Immune Evasion","volume":"10","author":"DeVito N. C.","year":"2019","journal-title":"Frontiers in Immunology"},{"issue":"7802","key":"e_1_2_13_84_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/s41586-020-2134-y","article-title":"A Conserved Dendritic\u2010Cell Regulatory Program Limits Antitumour Immunity","volume":"580","author":"Maier B.","year":"2020","journal-title":"Nature"},{"issue":"1","key":"e_1_2_13_85_1","doi-asserted-by":"crossref","first-page":"8151","DOI":"10.1038\/s41467-025-62878-5","article-title":"Mature and Migratory Dendritic Cells Promote Immune Infiltration and Response to Anti\u2010PD\u20101 Checkpoint Blockade in Metastatic Melanoma","volume":"16","author":"Yang J.","year":"2025","journal-title":"Nature Communications"},{"issue":"6657","key":"e_1_2_13_86_1","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1126\/science.ade2292","article-title":"CXCL9: SPP1 Macrophage Polarity Identifies a Network of Cellular Programs That Control Human Cancers","volume":"381","author":"Bill R.","year":"2023","journal-title":"Science"},{"issue":"3","key":"e_1_2_13_87_1","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cell.2021.01.010","article-title":"A Pan\u2010Cancer Single\u2010Cell Transcriptional Atlas of Tumor Infiltrating Myeloid Cells","volume":"184","author":"Cheng S.","year":"2021","journal-title":"Cell"},{"issue":"1","key":"e_1_2_13_88_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-020-17135-2","article-title":"B Cell Zone Reticular Cell Microenvironments Shape CXCL13 Gradient Formation","volume":"11","author":"Cosgrove J.","year":"2020","journal-title":"Nature Communications"},{"issue":"11","key":"e_1_2_13_89_1","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1038\/nri3298","article-title":"HEVs, Lymphatics and Homeostatic Immune Cell Trafficking in Lymph Nodes","volume":"12","author":"Girard J.\u2010P.","year":"2012","journal-title":"Nature Reviews Immunology"},{"issue":"12","key":"e_1_2_13_90_1","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1038\/s43018-021-00272-y","article-title":"Melanoma\u2010Derived Small Extracellular Vesicles Induce Lymphangiogenesis and Metastasis Through an NGFR\u2010Dependent Mechanism","volume":"2","author":"Garc\u00eda\u2010Silva S.","year":"2021","journal-title":"Nature Cancer"},{"issue":"4","key":"e_1_2_13_91_1","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.ccell.2025.12.019","article-title":"IFN\u03b3\u2010Dependent Metabolic Reprogramming Restrains an Immature, Pro\u2010Metastatic Lymphatic State in Melanoma","volume":"44","author":"Karakousi T.","year":"2026","journal-title":"Cancer Cell"},{"issue":"6576","key":"e_1_2_13_92_1","doi-asserted-by":"crossref","DOI":"10.1126\/science.abf9419","article-title":"Tertiary Lymphoid Structures in Cancer","volume":"375","author":"Schumacher T. N.","year":"2022","journal-title":"Science"},{"issue":"7791","key":"e_1_2_13_93_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/s41586-019-1914-8","article-title":"Tertiary Lymphoid Structures Improve Immunotherapy and Survival in Melanoma","volume":"577","author":"Cabrita R.","year":"2020","journal-title":"Nature"},{"issue":"7","key":"e_1_2_13_94_1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1038\/s41571-022-00619-z","article-title":"B Cells and Tertiary Lymphoid Structures as Determinants of Tumour Immune Contexture and Clinical Outcome","volume":"19","author":"Fridman W. H.","year":"2022","journal-title":"Nature Reviews Clinical Oncology"},{"issue":"13","key":"e_1_2_13_95_1","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.1200\/JCO.2004.12.009","article-title":"Prognostic Significance of Molecular Upstaging of Paraffin\u2010Embedded Sentinel Lymph Nodes in Melanoma Patients","volume":"22","author":"Takeuchi H.","year":"2004","journal-title":"Journal of Clinical Oncology"},{"issue":"1","key":"e_1_2_13_96_1","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1097\/SLA.0b013e3181fca894","article-title":"Molecular Upstaging Based on Paraffin\u2010Embedded Sentinel Lymph Nodes: Ten\u2010Year Follow\u2010Up Confirms Prognostic Utility in Melanoma Patients","volume":"253","author":"Nicholl M. B.","year":"2011","journal-title":"Annals of Surgery"},{"issue":"1","key":"e_1_2_13_97_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jaad.2017.08.039","article-title":"Determination of the Impact of Melanoma Surgical Timing on Survival Using the National Cancer Database","volume":"78","author":"Conic R. Z.","year":"2018","journal-title":"Journal of the American Academy of Dermatology"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70376","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.70376","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.70376","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:08:23Z","timestamp":1792044503000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.70376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":96,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/exd.70376"],"URL":"https:\/\/doi.org\/10.1111\/exd.70376","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70376"}}