{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T12:22:56Z","timestamp":1769170976516,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T00:00:00Z","timestamp":1372032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>altheria indica<\/jats:italic> is used in traditional pharmacopeia in <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>urkina <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>aso for the treatment of asthma and conditions of inflammation. To evaluate its pharmacological properties and isolate the active compounds, a study through a bioguided phytochemical approach was conducted. This search was guided by a two\u2010level investigation. First, we evaluated the impact of various fractions on the activity of enzymes involved in smooth muscle contraction (<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>4<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>1\u03b1) and inflammatory processes (<jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content><jats:sub>2<\/jats:sub>, 5\u2010<jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>). Second, we investigated the inhibitory effect of fractions on isolated rat trachea. The initial hydroalcoholic extract from roots of <jats:italic><jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>.\u00a0indica<\/jats:italic> (<jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>), n\u2010hexane fraction (F<jats:sub>1<\/jats:sub>), dichloromethane fraction (F<jats:sub>2<\/jats:sub>), ethyl acetate fraction (F<jats:sub>3<\/jats:sub>), residuary fraction (F<jats:sub>4<\/jats:sub>) reduced enzyme activity of PDE4A1<jats:italic>\u03b1<\/jats:italic> (inhibition of 22\u201342% at 50\u00a0\u03bcg\/mL)<jats:italic>,<\/jats:italic> 5\u2010LOX (60\u201380% at 10\u00a0\u03bcg\/mL), and <jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content><jats:sub>2<\/jats:sub> (42\u201394% at 100\u00a0\u03bcg\/mL). On isolated rat trachea, only <jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content>, F<jats:sub>3<\/jats:sub>, and fractions obtained from F<jats:sub>3<\/jats:sub> by chromatography on silica gel column, using dichloromethane\/methanol, dose dependently inhibited contraction induced by acetylcholine. <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub> was 1051\u00a0\u03bcg\/mL for <jats:styled-content style=\"fixed-case\">HA<\/jats:styled-content> and comprised between 181 and 477\u00a0\u03bcg\/mL for F<jats:sub>3<\/jats:sub> and its fractions. The most active fractions were purified and led to the identification of (\u2010)\u2010epicatechin. (\u2010)\u2010epicatechin from <jats:italic><jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>.\u00a0indica<\/jats:italic> dose dependently inhibited PLA<jats:sub>2<\/jats:sub> (<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub>\u00a0=\u00a0154.7\u00a0\u03bc<jats:sc>m<\/jats:sc>) and 5\u2010LOX (IC<jats:sub>50<\/jats:sub>\u00a0=\u00a015.8\u00a0\u03bc<jats:sc>m<\/jats:sc>). In conclusion, both inhibition of <jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>4<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>1<jats:italic>\u03b1<\/jats:italic>, 5\u2010<jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content><jats:sub>2<\/jats:sub> activities and rat trachea relaxation by <jats:italic><jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>.\u00a0indica<\/jats:italic> validate its use in traditional management of asthma and other conditions of inflammation. These effects should be, at least in part, attributed to the presence of (\u2010)\u2010epicatechin in roots of <jats:italic><jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>.\u00a0indica<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/fcp.12037","type":"journal-article","created":{"date-parts":[[2013,5,18]],"date-time":"2013-05-18T06:03:07Z","timestamp":1368856987000},"page":"323-330","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Bioguidage search of active compounds from <i><scp>W<\/scp>altheria indica <\/i><scp>L<\/scp>. (<scp>M<\/scp>alvaceae) used for asthma and inflammation treatment in <scp>B<\/scp>urkina <scp>F<\/scp>aso"],"prefix":"10.1111","volume":"28","author":[{"given":"Frank","family":"Zongo","sequence":"first","affiliation":[{"name":"Laboratoire HP2 \u2010 Inserm U1042 Joseph Fourier University BP 38706 La Tronche Grenoble France"},{"name":"Laboratory of Pharmaco\u2010Toxicology University of Ouagadougou I 03 BP 7021 Ougadougou Burkina Faso"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Ribuot","sequence":"additional","affiliation":[{"name":"Laboratoire HP2 \u2010 Inserm U1042 Joseph Fourier University BP 38706 La Tronche Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahc\u00e8ne","family":"Boumendjel","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry UMR CNRS 5063\u2010 Joseph Fourier University BP 38041 Grenoble France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Innocent","family":"Guissou","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaco\u2010Toxicology University of Ouagadougou I 03 BP 7021 Ougadougou Burkina Faso"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,24]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07030555"},{"key":"e_1_2_15_3_1","volume-title":"WHO traditional medicinal strategy 2002\u20132005","author":"World Health Organization.","year":"2002"},{"key":"e_1_2_15_4_1","volume-title":"M\u00e9decine et magie Africaine ou comment le noir se soigne t\u2010il?","author":"Traor\u00e9 D.","year":"1983"},{"key":"e_1_2_15_5_1","first-page":"24","article-title":"Traitement traditionnel de quelques maladies en pays Bissa (R\u00e9publique de Haute\u2010Volta)","volume":"1","author":"Sita G.","year":"1978","journal-title":"Bulletin Agricole du Rwanda."},{"key":"e_1_2_15_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.08.047"},{"key":"e_1_2_15_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.02.009"},{"key":"e_1_2_15_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.110.166967"},{"key":"e_1_2_15_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324797"},{"key":"e_1_2_15_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90022-5"},{"key":"e_1_2_15_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2010.00918.x"},{"key":"e_1_2_15_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.75879"},{"key":"e_1_2_15_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2010.00892.x"},{"key":"e_1_2_15_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.01.013"},{"key":"e_1_2_15_15_1","first-page":"28","article-title":"Relaxant effects of Ocimum basilicum on guinea pig tracheal chains and its possible mechanism(s)","volume":"13","author":"Mohammad H.B.","year":"2005","journal-title":"Daru."},{"key":"e_1_2_15_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.278.2.L320"},{"key":"e_1_2_15_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(01)00320-8"},{"key":"e_1_2_15_18_1","first-page":"79","article-title":"Bronchodilatory activity of Vitis vinifera leaf Hydroalcoholic extract in rat","volume":"10","author":"Kazem M.","year":"2006","journal-title":"Iran. 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