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Mitochondrial function is involved in modulating apoptosis of articular chondrocytes. This study was performed to investigate the mechanism of AGE<\/jats:styled-content>s\u2010induced chondrocyte apoptosis. The ratio of apoptotic cell and cell viability was surveyed by TUNEL<\/jats:styled-content>, MTT<\/jats:styled-content>,LDH<\/jats:styled-content> release assay. The reactive oxygen species was determined by the fluorescent probe 2\u2019, 7\u2019\u2010dichlorofluorescein diacetate. The expression of caspase\u20103 and cytochrome c was detected by Western blot. The mitochondrial membrane potential (\u25b5\u03a8m) was evaluated by rhodamine\u2010123 fluorescence. We found that AGE<\/jats:styled-content>s induced apoptosis in primary rabbit chondrocytes, upregulation of ROS<\/jats:styled-content> production, cytochrome c, and caspase\u20103 levels. Simultaneously, AGE<\/jats:styled-content>s decreases the levels of \u25b5\u03a8m and ATP<\/jats:styled-content> production; however, the antibody of AGE<\/jats:styled-content>s (sRAGE<\/jats:styled-content>) and antioxidant\u2010N\u2010acetylcys\u2010teine (NAC<\/jats:styled-content>) significantly reversed AGE<\/jats:styled-content>s\u2010induced the above damage thus to protect the cells from apoptosis. These observations suggested that the mechanism of AGE<\/jats:styled-content>s\u2010induced chondrocyte apoptosis was primarily via ROS<\/jats:styled-content> production and mitochondria\u2010mediated caspase\u20103 activation.<\/jats:p>","DOI":"10.1111\/fcp.12094","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T09:01:55Z","timestamp":1412586115000},"page":"54-61","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Advanced glycation end products\u2010induced chondrocyte apoptosis through mitochondrial dysfunction in cultured rabbit chondrocyte"],"prefix":"10.1111","volume":"29","author":[{"given":"Qingshan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Gan Su Province Hospital Lan Zhou 73000 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