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Here, we studied the effect of H\u201089 (protein kinase A inhibitor), bucladesine (Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>) (membrane\u2010permeable analog of<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>), and pentoxifylline (<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>; nonspecific phosphodiesterase (<jats:styled-content style=\"fixed-case\">PDE<\/jats:styled-content>) inhibitor) on pain sensation. Different doses of H\u201089 (0.05, 0.1, and 0.5 mg\/100 g),<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>(5, 10, and 20 mg\/100 g), and Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>(50, 100, and 300 n<jats:sc>m<\/jats:sc>\/mouse) were administered intraperitoneally (I.p.) 15 min before a tail\u2010flick test. In combination groups, we injected the first and the second compounds 30 and 15 min before the tail\u2010flick test, respectively. I.p. administration of H\u201089 and<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>significantly decreased the thermal\u2010induced pain sensation in their low applied doses. Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>, however, decreased the pain sensation in a dose\u2010dependent manner. The highest applied dose of H\u201089 (0.5 mg\/100 g) attenuated the antinociceptive effect of Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>in doses of 50 and 100 n<jats:sc>m<\/jats:sc>\/mouse. Surprisingly, Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>decreased the antinociceptive effect of the lowest dose of H\u201089 (0.05 mg\/100 g). All applied doses of<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>reduced the effect of 0.05 mg\/100 g H\u201089 on pain sensation; however, the highest dose of H\u201089 compromised the antinociceptive effect of 20 mg\/100 g dose of<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>. Co\u2010administration of Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>increased the antinociceptive effect of each compound on thermal\u2010induced pain. In conclusion,<jats:styled-content style=\"fixed-case\">PTX<\/jats:styled-content>, H\u201089, and Db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>affect the thermal\u2010induced pain by probably interacting with intracellular<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content>signaling pathways and cyclic nucleotide\u2010dependent protein kinases.<\/jats:p>","DOI":"10.1111\/fcp.12282","type":"journal-article","created":{"date-parts":[[2017,3,7]],"date-time":"2017-03-07T19:56:40Z","timestamp":1488916600000},"page":"411-419","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effect of bucladesine, pentoxifylline, and H\u201089 as cyclic adenosine monophosphate analog, phosphodiesterase, and protein kinase A inhibitor on acute pain"],"prefix":"10.1111","volume":"31","author":[{"given":"Forouz","family":"Salehi","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Pharmaceutical Science Research Center Tehran University of Medical Science PO Box 14155\u20106451 Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9202-6472","authenticated-orcid":false,"given":"Mahshid S.","family":"Hosseini\u2010Zare","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Pharmaceutical Science Research Center Tehran University of Medical Science PO Box 14155\u20106451 Tehran Iran"},{"name":"Department of Biology and Biochemistry University of Houston Houston TX 77204 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haleh","family":"Aghajani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston Houston TX 77204 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyedeh Yalda","family":"Seyedi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Pharmaceutical Science Research Center Tehran University of Medical Science PO Box 14155\u20106451 Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam S.","family":"Hosseini\u2010Zare","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston Houston TX 77204 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Sharifzadeh","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Pharmaceutical Science Research Center Tehran University of Medical Science PO Box 14155\u20106451 Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00534-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(01)00340-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1054\/jpai.2002.123653"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2393-7-S1-S10"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2000.0360"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.18.12107"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"6989","DOI":"10.1523\/JNEUROSCI.20-18-06989.2000","article-title":"Enhanced phosphorylation of NMDA receptor 1 subunits in spinal cord dorsal horn and spinothalamic tract neurons after intradermal injection of capsaicin in rats","volume":"15","author":"Zou X.","year":"2000","journal-title":"J. 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