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The diabetic model (<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>) was induced through the administration of 10% fructose in drinking water for 2 weeks followed by streptozotocin injection (<jats:italic>intraperitoneal<\/jats:italic>). One week later, hyperglycemia was assisted and diabetic animals were treated with <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> either as local eye drops (0.5% solution, four times\/day) or orally (100 mg\/kg b.w.). Cataract formation was monitored biweekly and scored into four stages. After 12 weeks of treatment, rats were subjected to ophthalmological examination, and then, their blood and lenses were prepared for biochemical analysis of glucose, insulin, reduced glutathione, total antioxidant capacity, malondialdehyde, hydrogen peroxide, caspase\u201012, and lenticular total proteins. In addition, tertiary structure and conformational changes of lenticular soluble proteins were analyzed using <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> absorption while changes in lenticular \u03b1\u2010crystallin structure were investigated using intrinsic tryptophan fluorescence. Results demonstrated that both local and oral <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> restored the normal levels of lens <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">AOC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MDA<\/jats:styled-content>, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, and caspase\u201012 and improved noticeably the levels of the lens <jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content> and total proteins. In addition, conformational and tertiary structure changes of soluble lens proteins were significantly reduced in <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content>\u2010treated groups. Morphological examination of lenses revealed decreased score of cataract progression in <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content>\u2010treated groups compared to <jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content> animals. It was concluded that <jats:styled-content style=\"fixed-case\">UDCA<\/jats:styled-content> decreased the incidence of diabetic cataract by maintaining the antioxidant status, reducing the endoplasmic reticulum stress, and suppressing the structural changes of soluble lens proteins.<\/jats:p>","DOI":"10.1111\/fcp.12385","type":"journal-article","created":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T15:46:00Z","timestamp":1528127160000},"page":"627-640","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ursodeoxycholic acid suppresses the formation of fructose\/streptozotocin\u2010induced diabetic cataract in rats"],"prefix":"10.1111","volume":"32","author":[{"given":"Amany","family":"Abdel\u2010Ghaffar","sequence":"first","affiliation":[{"name":"Unit of Biochemistry and Pharmacology Research Institute of Ophthalmology El Ahram st Giza 12557 Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hala M.","family":"Ghanem","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science Ain Shams University Khalifa El\u2010Maamon st, Abbasiya sq. 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