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Precise treatment of CP\/CPPS is not available due to lack of specific cause; however, autoimmunity is the most valid theory. We develop a new treatment strategy that involves synthesis and coupling of biodegradable nanoparticles to antigenic T2 peptide to induce immune tolerance in CP\/CPPS mice models. A total of 50 male C57BL\/6 mice were randomized into five groups, that is, na\u00efve, Model, PLGA\u2010PEMA, PLGA\u2010PEMA\u2010OVA<jats:sub>323\u2010339<\/jats:sub>, and PLGA\u2010PEMA\u2010T2 group. All groups except na\u00efve were injected subcutaneously on day 0 with 0.2\u00a0mL of T2 peptide with CFA to generate valid CP\/CPPS models. After successful induction of CP\/CPPS, Model group, PLGA\u2010PEMA, PLGA\u2010PEMA\u2010OVA, and PLGA\u2010PEMA\u2010T2 groups were treated with 0.15\u00a0mL of normal saline, 0.2\u00a0mg of PLGA\u2010PEMA and PLG\u2010PEMA\u2010T2 and 0.3\u00a0mg PLGA\u2010PEMA\u2010OVA nanoparticles, respectively, on day 28. Hematoxylin and eosin staining, and ELISA were used to evaluate the variation in CP\/CPPS manifestations and seral level of IL\u201010 in each group. Pain threshold and voiding behavior were also recorded for every group. Mice treated with PLGA\u2010PEMA\u2010T2 exhibited enhanced pain threshold, reduced urine frequency, and prostate pathology. Furthermore, serum level of inflammatory mediators (TNF\u2010\u03b1 and CRP) were reduced and anti\u2010inflammatory IL\u201010 was enhanced in PLGA\u2010PEMA\u2010T2 group as compared to other groups. Our results demonstrate that PLGA\u2010PEMA\u2010T2 nanoparticle ameliorates disease manifestations in CP\/CPPS mice models and upregulates IL\u201010 which is essential for tolerance induction. This strategy highlights the new therapeutic approach utilizing biodegradable nanoparticles for the treatment of CP\/CPPS.<\/jats:p>","DOI":"10.1111\/fcp.12438","type":"journal-article","created":{"date-parts":[[2018,11,24]],"date-time":"2018-11-24T17:00:19Z","timestamp":1543078819000},"page":"267-276","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A nanoparticle\u2010coupled T2 peptide induces immune tolerance and ameliorates chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS) in mice model"],"prefix":"10.1111","volume":"33","author":[{"given":"Yanfang","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacy School of Basic Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing Jiangsu Province 211198 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijie","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department 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