{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:09:19Z","timestamp":1773277759224,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600767"],"award-info":[{"award-number":["81600767"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Retinal degeneration (<jats:styled-content style=\"fixed-case\">RD<\/jats:styled-content>) results in photoreceptor loss and irreversible visual impairments. This study sought to alleviate the photoreceptor degeneration via the adeno\u2010associated virus (<jats:styled-content style=\"fixed-case\">AAV<\/jats:styled-content>)\u2010mediated erythropoietin (<jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content>) therapy. <jats:styled-content style=\"fixed-case\">AAV<\/jats:styled-content>\u20102\/2\u2010<jats:styled-content style=\"fixed-case\">mCMV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content> vectors were constructed and delivered into the subretinal space of a <jats:styled-content style=\"fixed-case\">RD<\/jats:styled-content> model. The retinal morphology, optokinetic behaviour and electrophysiological function of the treated animals were analysed. The subretinal delivery of <jats:styled-content style=\"fixed-case\">AAV<\/jats:styled-content>\u20102\/2 vectors induced robust <jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content> gene expressions in the retinas. <jats:styled-content style=\"fixed-case\">AAV<\/jats:styled-content>2\/2\u2010mediated <jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content> therapy ameliorated the photoreceptor degeneration and visual impairments of the <jats:styled-content style=\"fixed-case\">RD<\/jats:styled-content> animal model. Furthermore, the multi\u2010electrodes array (<jats:styled-content style=\"fixed-case\">MEA<\/jats:styled-content>) was used to detect the firing activities of retinal ganglion cells. <jats:styled-content style=\"fixed-case\">MEA<\/jats:styled-content> recording showed that the <jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content> therapy could restrain the spontaneous firing response, enhance the light\u2010induced firing response and preserve the basic configurations of visual signal pathway in <jats:styled-content style=\"fixed-case\">RD<\/jats:styled-content> model. Our <jats:styled-content style=\"fixed-case\">MEA<\/jats:styled-content> assay provided an example to evaluate the potency of pharmacological compounds on retinal plasticity. In conclusion, <jats:styled-content style=\"fixed-case\">AAV<\/jats:styled-content>2\/2\u2010mediated <jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content> therapy can ameliorate the photoreceptor degeneration and rectify the abnormities in visual signal transmission. These beneficial results suggest the <jats:styled-content style=\"fixed-case\">AAV<\/jats:styled-content> vector is a viable therapeutic option for retinopathies with rapidly degenerating kinetics and lay the groundwork for future development of <jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content> gene therapy.<\/jats:p>","DOI":"10.1111\/fcp.12494","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T08:33:17Z","timestamp":1561624397000},"page":"131-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Adeno\u2010associated virus (<scp>AAV<\/scp>)\u2010mediated neuroprotective effects on the degenerative retina: the therapeutic potential of erythropoietin"],"prefix":"10.1111","volume":"34","author":[{"given":"Ye","family":"Tao","sequence":"first","affiliation":[{"name":"Department of Ultrasound China\u2010Japan Union Hospital of Jilin University Changchun 130031 China"},{"name":"Department of Ophthalmology Key Lab of Ophthalmology and Visual Science Chinese PLA General Hospital Beijing 100853 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology The Second Hospital of Jilin University Changchun 130041 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Key Lab of Ophthalmology and Visual Science Chinese PLA General Hospital Beijing 100853 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaobing","family":"Zha","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Zhongshan Hospital Fudan University Shanghai 200032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dengke","family":"Teng","sequence":"additional","affiliation":[{"name":"Department of Ultrasound China\u2010Japan Union Hospital of Jilin University Changchun 130031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5830-5710","authenticated-orcid":false,"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery China\u2010Japan Union Hospital of Jilin University Changchun 130033 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00347-002-0633-x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.1086"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0750-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652409787847155"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00469"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.12.034"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/fcp.12226"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2005.00347.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369885"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr115"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1288-04.2004"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18612"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2018.1389886"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.171"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2008.66"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.269"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652310793180670"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6138"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"e0136523","DOI":"10.1371\/journal.pone.0136523","article-title":"Trans\u2010corneal subretinal injection in mice and its effect on the function and morphology of the retina","volume":"28","author":"Qi Y.","year":"2015","journal-title":"PLoS ONE"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011306"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.2000.0921"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2017.10.007"},{"key":"e_1_2_8_24_1","first-page":"933","article-title":"Animal models for retinitis pigmentosa induced by MNU; disease progression, mechanismand therapeutic trials","volume":"25","author":"Tsubura A.","year":"2010","journal-title":"Histol. 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