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To better investigate the effects of LQFM030 regarding the p53 mutation status, this study investigated the antiproliferative activity of LQFM030 against the p53\u2010null K562 leukemia cells as well as the cell death pathways involved. In addition, the effects of LQFM030 on the levels of the p53\/MDM2 complex were also carried out using 3T3 cells as a p53 wild\u2010type model. Our data suggest that LQFM030 triggered apoptosis in K562 cells via different mechanisms including cell cycle arrest, caspase activation, reduction of mitochondrial activity, decrease in MDM2 expression, and transcriptional modulation of MDMX, p73, MYC, and NF\u2010\u0138B. Additionally, it promoted effects in p53\/MDM2 binding in p53 wild\u2010type 3T3 cells. Therefore, LQFM030 has antiproliferative effects in cancer cells by a p53 mutation status\u2010independent manner with different signaling pathways. These findings open new perspectives to the treatment of leukemic cells considering the resistance development associated with cancer treatment with conventional cytotoxic drugs.<\/jats:p>","DOI":"10.1111\/fcp.12540","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T10:06:40Z","timestamp":1580724400000},"page":"444-457","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Small\u2010molecule MDM2 inhibitor LQFM030\u2010induced apoptosis in p53\u2010null K562 chronic myeloid leukemia cells"],"prefix":"10.1111","volume":"34","author":[{"given":"Higor","family":"de Oliveira Ribeiro","sequence":"first","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology \u2013 Tox In Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alane Pereira","family":"Cortez","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology \u2013 Tox In Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato Ivan","family":"de \u00c1vila","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology \u2013 Tox In Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artur Christian Garcia","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology \u2013 Tox In Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio Silva","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Farmac\u00eautica Medicinal (LQFM) Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Menegatti","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Qu\u00edmica Farmac\u00eautica Medicinal (LQFM) Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano Morais","family":"Li\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Resson\u00e2ncia Magn\u00e9tica Nuclear Instituto de Qu\u00edmica Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0379-1325","authenticated-orcid":false,"given":"Marize Campos","family":"Valadares","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology \u2013 Tox In Faculty of Pharmacy Universidade Federal de Goi\u00e1s Goi\u00e2nia 74605-220 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.10.3343"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-010-0016-z"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/2040620716657994"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-018-0665-2"},{"key":"e_1_2_6_6_1","first-page":"42","article-title":"Tyrosine kinase inhibitors for the treatment of chronic\u2010phase chronic myeloid leukemia: long\u2010term patient care and management","volume":"7","author":"Bauer S.","year":"2016","journal-title":"J. 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