{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:15:27Z","timestamp":1770999327145,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this work was to explore whether bisoprolol plays a protective role in cardiomyocytes against ischemia\u2013reperfusion injury via PI3K\/AKT\/ GSK3\u03b2 pathway. We pretreated male Sprague Dawley (SD) rats with bisoprolol by oral administration prior to 0.5\u00a0h ischemia\/4\u00a0h reperfusion. Myocardial infarct size and serum levels of cTnI and CK\u2010MB were measured. In vitro, H9c2 cells were treated with hypoxia and reoxygenation, followed by measurement of cell viability, apoptosis, ROS production, cytometry, activities of AKT, GSK3\u03b2, and p\u201038 in the presence and absence of GSK3\u03b2 siRNA. We found that bisoprolol reduced infarct size from 44% in I\/R group to 31% in treated group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). The levels of cTnI and CK\u2010MB were decreased from 286\u00a0\u00b1\u00a07\u00a0pg\/mL and 32.2\u00a0\u00b1\u00a02\u00a0ng\/mL in I\/R group to 196\u00a0\u00b1\u00a02\u00a0pg\/mL and 19.6\u00a0\u00b1\u00a00.9\u00a0ng\/mL in the treated group, respectively (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Bisoprolol also increased cell viability while decreased apoptosis and ROS production in the treatment of hypoxia\/ reoxygenation. Furthermore, bisoprolol increased AKT and GSK3\u03b2 phosphorylation, an effect that was immediately eliminated by LY294002. GSK3\u03b2\u2010specific siRNA experiment further confirmed that bisoprolol protected the myocardium against hypoxia\/reoxygenation\u2010induced injury via suppressing GSK3\u03b2 activity. In conclusion, bisoprolol protected myocardium against ischemia\u2013reperfusion injury via the PI3K\/AKT\/ GSK3\u03b2 pathway.<\/jats:p>","DOI":"10.1111\/fcp.12562","type":"journal-article","created":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T05:42:53Z","timestamp":1588570973000},"page":"708-720","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Bisoprolol, a \u03b2<sub>1<\/sub> antagonist, protects myocardial cells from ischemia\u2013reperfusion injury via PI3K\/AKT\/GSK3\u03b2 pathway"],"prefix":"10.1111","volume":"34","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiology Jinling Hospital Nanjing University School of Medicine 305 East Zhongshan Rd. 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Nanjing 210002 Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cardiology Jinling Hospital Nanjing University School of Medicine 305 East Zhongshan Rd. Nanjing 210002 Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbin","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Cardiology Jinling Hospital Nanjing University School of Medicine 305 East Zhongshan Rd. 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